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Patroklos Vareltzis Irene Gargali Smaro Kiroglou Marina Zeleskidou


This study aimed to develop a process for producing instant coffee from cold brewed Arabica and Robusta roasted coffee beans. The process starts by selecting an appropriate mixture of Arabica and Robusta coffee beans, degree of roasting (light, medium, dark) and degree of grinding (coarse, fine). Sensory evaluation by a trained panel showed that a mixture of 92.5% Arabica and 7.5% Robusta medium roasted, coarsely ground coffee beans produces instant cold brewed coffee equally or better accepted from other instant coffees available to consumers. The selected coffee beans were subjected to vacuum assisted cold extraction and the extract was spray dried. Spray drying conditions (inlet temperature, air volumetric flow and air aspiration rate) were optimized by a 33 full factorial design. Response variables were solids yield, process thermal efficiency and anti-oxidant capacity of the produced powder measured as %DPPH scavenging ability, FRAP and total phenolic content. The produced powder was analysed for its physicochemical characteristics (caffeine content, phenolics, moisture content, hygroscopicity, bulk density, solubility, particle size distribution, acrylamide content and shelf life determination).

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Aguiar, J., Estevinho, B. N., & Santos, L. (2016). Microencapsulation of natural antioxidants for food application – The specific case of coffee antioxidants – A review. Trends in Food Science & Technology, 58, 21–39

Bagdonaite, K, & Murkovic, M. (2004). Factors affecting the formation of acrylamide in coffee. In Chemical Reaction in Food (pp. 22–25). Prague: Chech J Food Sci.

Bagdonaite, Kristina, Derler, K., & Murkovic, M. (2008). Determination of Acrylamide during Roasting of Coffee. Journal of Agricultural and Food Chemistry, 56(15), 6081–6086

Bansal, V., Sharma, H. K., & Nanda, V. (2014). Optimisation of spray drying process parameters for low-fat honey-based milk powder with antioxidant activity. International Journal of Food Science and Technology, 49(4), 1196–1202.

Bertrand, B., Villarreal, D., Laffargue, A., Posada, H., Lashermes, P., & Dussert, S. (2008). Comparison of the Effectiveness of Fatty Acids, Chlorogenic Acids, and Elements for the Chemometric Discrimination of Coffee (Coffea arabica L.) Varieties and Growing Origins. Journal of Agricultural and Food Chemistry, 56(6), 2273–2280.

Burmester, K., Pietsch, A., & Eggers, R. (2012). A basic investigation on instant coffee production by vacuum belt drying. Procedia Food Science.

Butt, M. S., & Sultan, M. T. (2011). Coffee and its Consumption: Benefits and Risks. Critical Reviews in Food Science and Nutrition, 51(4), 363–373.

Chandrasekar, V., & Viswanathan, ; R. (1999). Physical and Thermal Properties of Coffee. J. Agric. Engng Res (Vol. 73).

Chu, Y.-F. (2012). Coffee : emerging health effects and disease prevention. (Y.-F. Chu, Ed.). IFT.

Cordoba, N., Pataquiva, L., Osorio, C. et al. (2019). Effect of grinding, extraction time and type of coffee on the physicochemical and flavour characteristics of cold brew coffee. Sci Rep 9, 8440

Denis Andrzejewski, *, John A. G. Roach, Martha L. Gay, and, & Musser, S. M. (2004). Analysis of Coffee for the Presence of Acrylamide by LC-MS/MS.

Fuller, M., & Rao, N. Z. (2017). The Effect of Time, Roasting Temperature, and Grind Size on Caffeine and Chlorogenic Acid Concentrations in Cold Brew Coffee OPEN, 7, 17979

Goula, A. M., Adamopoulos, K. G., & Kazakis, N. A. (2004). Influence of spray drying conditions on tomato powder properties. Drying Technology, 22(5), 1129–1151

Grembecka, M., Malinowska, E., & Szefer, P. (2007). Differentiation of market coffee and its infusions in view of their mineral composition. Science of The Total Environment, 383(1–3), 59–69

Hudáková, J., Marcinčáková, D., and Legáth, J.(2016). Study of antioxidant effects of selected types of coffee. Folia Veterinaria, 60(3), 34–38

Kreuml, M. T. L., Majchrzak, D., Ploederl, B., & Koenig, J. (2013). Changes in sensory quality characteristics of coffee during storage. Food Science & Nutrition, 1(4), 267–272c

MacLeod, C. S., McKittrick, J. A., Hindmarsh, J. P., Johns, M. L., & Wilson, D. I. (2006). Fundamentals of spray freezing of instant coffee. Journal of Food Engineering, 74(4), 451–461

Manzocco, L., & Nicoli, M. C. (2007). Modeling the effect of water activity and storage temperature on chemical stability of coffee brews. Journal of Agricultural and Food Chemistry, 55(16), 6521–6526.

Margarita Pérez-Hernández, L., Chávez-Quiroz, K., Ángel Medina-Juárez, L., & Gámez Meza, N. (2012). Phenolic Characterization, Melanoidins, and Antioxidant Activity of Some Commercial Coffees from Coffea arabica and Coffea canephora Article. J. Mex. Chem. Soc (Vol. 56)

Mortas, M., Gul, O., Yazici, F., & Dervisoğlu, M. (2017). International Journal of Food Properties Effect of brewing process and sugar content on 5-hydroxymethylfurfural and related substances from Turkish coffee

Murkovic, M., & Pichler, N. (2006). Analysis of 5-hydroxymethylfurfual in coffee, dried fruits and urine. Molecular Nutrition & Food Research, 50(9), 842–846

Murugesan, R., & Orsat, V. (2011). Spray Drying of Elderberry ( Sambucus nigra L.) Juice to Maintain Its Phenolic Content. Drying Technology, 29(14), 1729–1740.

Nicoli, M. C., Calligaris, S., & Manzocco, L. (2009). Shelf-Life Testing of Coffee and Related Products: Uncertainties, Pitfalls, and Perspectives. Food Engineering Reviews, 1(2), 159–168

Rao, N. Z., & Fuller, M. (2018). Acidity and Antioxidant Activity of Cold Brew Coffee OPEN. Scientific RepoRts, 8, 16030

Ratti, C. (2001). Hot air and freeze-drying of high-value foods: a review. Journal of Food Engineering, 49(4), 311–319

How to Cite
VARELTZIS, Patroklos et al. Production of instant coffee from cold brewed coffee; process characteristics and optimization. Food Science and Applied Biotechnology, [S.l.], v. 3, n. 1, p. 39-46, mar. 2020. ISSN 2603-3380. Available at: <>. Date accessed: 24 june 2024. doi: