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Federica Tinello Dasha Mihaylova Anna Lante

Abstract

The enzymatic browning, whose main responsible is polyphenol oxidase (PPO, EC 1.14.18.1), is involved in the phenolic oxidation and colour alteration of minimally-processed fruits and vegetables. Currently, the research of new strategies to inactivate PPO is moving towards replacing synthetic additives such as organic acids and sulphites with natural inhibitors. The present study is focused on investigating the anti-browning performance of juices and distillates obtained from three onion varieties (white, yellow, and red) and Borettana onion wastes (inner layers). Their inhibitory activity on a commercial mushroom tyrosinase and some plant PPOs has been evaluated by spectrophotometric and electrophoretic analysis. The in vivo trials has been also carried out by monitoring over time at room temperature the colour change on potato slices under accelerated browning conditions. The effectiveness of onion samples in limiting enzymatic browning was affected by not only the enzyme source but also inhibitor type. Although distillates had higher anti-PPO capacity as confirmed by in vitro assays, juices showed better in vivo effectiveness. Hence, onions and their wastes can be valorised as a natural source of anti-browning agents to control PPO activity thus preserving sensory, antioxidant and nutritional properties of agro-food products.

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References

Benítez V., Mollá E., Martín-Cabrejas M.A., Aguilera Y., López-Andréu F.J., Cools K., Terry L.A., Esteban R.M. (). Characterization of industrial onion wastes (Allium cepa L.): dietary fibre and bioactive compounds. Plant Foods for Human Nutrition, 2011, 66(1): 48-57 https://doi.org/10.1007/s11130-011-0212-x

Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein uilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3

Corzo-Martínez M., Corzo N., Villamiel, M. Biological properties of onions and garlic. Trends in Food Science & Technology, 2007, 18(12): 609-625 https://doi.org/10.1016/j.tifs.2007.07.011

Lante A., Nardi T., Zocca F., Giacomini A., Corich V. Evaluation of red chicory extract as a natural antioxidant by pure lipid oxidation and yeast oxidative stress response as model systems. Journal of Agricultural and Food Chemistry, 2011, 59(10): 5318-5324. https://doi.org/10.1021/jf2003317

Lante A., Tinello F. Citrus hydrosols as useful by-products for tyrosinase inhibition. Innovative Food Science & Emerging Technologies, 2015, 27: 154-159. https://doi.org/10.1016/j.ifset.2014.11.001

Lante A., Tinello F., Lomolino G. Effect of UV light on microbial proteases: From enzyme inactivation to antioxidant mitigation. Innovative Food Science & Emerging Technologies, 2013, 17: 130-134. https://doi.org/10.1016/j.ifset.2012.11.002

Lante A., Tinello F., Lomolino G. The use of polyphenol oxidase activity to identify a potential raisin variety. Food Biotechnology, 2016, 30(2): 98-109. https://doi.org/10.1080/08905436.2016.1166125

Lante A., Tinello F., Nicoletto M. UV-A light treatment for controlling enzymatic browning of fresh-cut fruits. Innovative Food Science & Emerging Technologies, 2016, 34: 141-147 https://doi.org/10.1016/j.ifset.2015.12.029

Mihaylova D.S., Lante A., Tinello F., Krastanov A.I. Study on the antioxidant and antimicrobial activities of Allium ursinum L. pressurised-liquid extract. Natural Product Research, 2014, 28(22): 2000-2005. https://doi.org/10.1080/14786419.2014.923422

Pal, C.B.T., Jadeja G.C. Microwave-assisted deep eutectic solvent extraction of phenolic antioxidants from onion (Allium cepa L.) peel: a Box–Behnken design approach for optimization. Journal of Food Science and Technology, 2019, 56(9): 4211-4223 https://doi.org/10.1007/s13197-019-03891-7

Piechowiak T., Grzelak-Błaszczyk K., Bonikowski R., Balawejder M. Optimization of extraction process of antioxidant compounds from yellow onion skin and their use in functional bread production. LWT-Food Science and Technology, 2020, 117: 108614 https://doi.org/10.1016/j.lwt.2019.108614

Putnik P., Gabrić D., Roohinejad S., Barba F.J., Granato D., Mallikarjunan K., Lorenzo J.M., Kovačević D.B. An overview of organosulfur compounds from Allium spp.: From processing and preservation to evaluation of their bioavailability, antimicrobial, and anti-inflammatory properties. Food Chemistry, 2019, 276: 680-691. https://doi.org/10.1016/j.foodchem.2018.10.068

Roldán E., Sánchez-Moreno C., de Ancos B., Cano M.P. Characterisation of onion (Allium cepa L.) by-products as food ingredients with antioxidant and antibrowning properties. Food Chemistry, 2008, 108(3): 907-916. https://doi.org/10.1016/j.foodchem.2007.11.058

Santas J., Carbo R., Gordon M.H., Almajano M.P. Comparison of the antioxidant activity of two Spanish onion varieties. Food Chemistry, 2008, 107(3): 1210-1216 https://doi.org/10.1016/j.foodchem.2007.09.056

Sidhu J.S., Ali M., Al-Rashdan A., Ahmed N. Onion (Allium cepa L.) is potentially a good source of important antioxidants. Journal of Food Science and Technology, 2019, 56(4): 1811-1819 https://doi.org/10.1007/s13197-019-03625-9

Singh B.N., Singh B.R., Singh R.L., Prakash D., Singh D.P., Sarma B.K., Upadhyay G., Singh H.B. Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities. Food and Chemical Toxicology, 2009, 47(6): 1161-1167.

Tinello F., Lante A. Evaluation of antibrowning and antioxidant activities in unripe grapes recovered during bunch thinning. Australian Journal of Grape and Wine Research, 2017, 23: 33-41 https://doi.org/10.1111/ajgw.12256

Tinello F., Lante A. Recent advances in controlling polyphenol oxidase activity of fruit and vegetable products. Innovative Food Science & Emerging Technologies, 2018, 50: 73-83 https://doi.org/10.1016/j.ifset.2018.10.008

Tinello F., Lante A. Valorisation of ginger and turmeric peels as source of natural antioxidants. Plant Foods for Human Nutrition, 2019, 1-3 https://doi.org/10.1007/s11130-019-00748-4

Tinello F., Mihaylova D., Lante A. (2018) Effect of dipping pretreatment with unripe grape juice on dried “Golden delicious” apple slices. Food and Bioprocess Technology, 2018, 11: 2275-2285 https://doi.org/10.1007/s11947-018-2186-2

Tinello F., Vendramin V., Barros Divino V. Co-fermentation of onion and whey: a promising synbiotic combination. Journal of Functional Foods, 2017, 39: 233-237 https://doi.org/10.1016/j.jff.2017.10.018

Yuniarti T., Sukarno, Yuliana N.D., Budijanto S. Inhibition of enzymatic browning by onion (Allium cepa L.): investigation on inhibitory mechanism and identification of active compounds. Current Research in Nutrition and Food Science, 2018, 6(3): 770-780 https://doi.org/10.12944/CRNFSJ.6.3.19

Zocca F., Lomolino G., Lante A. . Antibrowning potential of Brassicacaea processing water. Bioresource Technology, 2010, 101(10): 3791-3795 https://doi.org/10.1016/j.biortech.2009.12.126

Zocca F., Lomolino G., Lante A. Dog rose and pomegranate extracts as agents to control enzymatic browning. Food Research International, 2011, 44(4): 957-963 https://doi.org/10.1016/j.foodres.2011.02.010

How to Cite
TINELLO, Federica; MIHAYLOVA, Dasha; LANTE, Anna. Valorization of onion extracts as anti-browning agents. Food Science and Applied Biotechnology, [S.l.], v. 3, n. 1, p. 16-21, mar. 2020. ISSN 2603-3380. Available at: <https://www.ijfsab.com/index.php/fsab/article/view/87>. Date accessed: 08 july 2020. doi: https://doi.org/10.30721/fsab2020.v3.i1.87.