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Gergana Sherova Atanas Pavlov Vasil Georgiev


In vitro plants of Capsicum chinense cv. Carolina Reaper have been obtained by planting seeds on sterile MS medium. The plants were used to initiate callus culture on half strength MS medium, supplied with 2 mg/l 1-Naphthaleneacetic acid and 0.5 mg/L 6-Benzylaminopurine. The polyphenol profiles (phenolic acids and flavonoids) of methanol extracts from obtained callus and in vitro plants have been analyzed by HPLC. The main constituents in plant extract were protocatechuic acid, sinapic acid, rutin, hesperetin and myricetin, whereas in callus extract the major compounds were found to be chlorogenic acid, ferulic acid, rutin, hyperoside, myricetin and hesperetin. The antioxidant capacity of both extracts have been evaluated by using DPPH, TEAC, FRAP and CUPRAC assays. In our knowledge, this is the first report for obtaining of callus culture from Capsicum chinense cv. Carolina Reaper and evaluation of phytochemical profiles and antioxidant activities of its extract. Practical applications: The research outlines the potential of Capsicum in vitro systems as a renewable source of active ingredients for application in cosmetic and food products.

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Aldana-Iuit, J. G., Sauri-Duch, E., Miranda-Ham, M. D. L., Castro-Concha, L. A., Cuevas-Glory, L. F., Vázquez-Flota, F. A. Nitrate promotes capsaicin accumulation in Capsicum chinense immobilized placentas. BioMed research international, 2015, (2015): 794084.

Antonio, A.S., Wiedemann, L.S.M., Veiga Junior, V.F. The genus Capsicum: a phytochemical review of bioactive secondary metabolites. RSC Advances. 2018, 8(45): 25767-25784.

Bae, H., Jayaprakasha, G.K., Crosby, K., Yoo, K.S., Leskovar, D.I., Jifon, J,, Patil, B.S. Ascorbic acid, capsaicinoid, and flavonoid aglycone concentrations as a function of fruit maturity stage in greenhouse-grown peppers. Journal of Food Composition and Analysis. 2014, 33(2): 195-202.


Chopan, M., Littenberg, B. The Association of Hot Red Chili Pepper Consumption and Mortality: A Large Population-Based Cohort Study. PloS one, 2017, 12(1): e0169876.

Dalton, P., Byrnes, N. Psychology of chemesthesis – why would anyone want to be in pain? In: Chemesthesis: Chemical Touch in Food and Eating, (S. T. McDonald, D. A. Bolliet, J. E. Hayes, Eds.), Wiley-Blackwell, 2016, pp. 8-32, ISBN- 978-1-118-95173-6.

Duelund, L., Mouritsen, O.G. Contents of capsaicinoids in chillies grown in Denmark. Food chemistry, 2017, 221: 913-918.

Ferri, M., Gruarin, N., Barbieri, F., Tassoni, A. Capsicum spp in vitro liquid cell suspensions: A useful system for the production of capsaicinoids and polyphenols. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, (2017): 1-9.

Haley, H., McDonald, S. T. Spice and herb extracts with chemesthetic effects. In: Chemesthesis: Chemical Touch in Food and Eating (S. T. McDonald, D. A. Bolliet, J. E. Hayes, Eds.), Wiley-Blackwell, 2016, pp. 32-48, ISBN- 978-1-118-95173-6.

Ivanov, I., Vrancheva, R., Marchev, A., Petkova, N., Aneva, I., Denev, P., Georgiev, V.G., Pavlov, A. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. Int. J. Curr. Microbiol. App. Sci, 2014, 3(2): 296-306. ISSN: 2319-7706

Kehie, M., Kumaria, S., Tandon, P. Biotechnological enhancement of capsaicin biosynthesis in cell suspension cultures of Naga King Chili (Capsicum chinense Jacq.). Bioprocess and biosystems engineering, 2016, 39(1): 205-210.

Kehie, M., Kumaria, S., Tandon, P. In vitro plantlet regeneration from nodal segments and shoot tips of Capsicum chinense Jacq. cv. Naga King Chili. 3 Biotech, 2012, 2(1): 31-35.

Kehie, M., Kumaria, S., Tandon, P. Manipulation of culture strategies to enhance capsaicin biosynthesis in suspension and immobilized cell cultures of Capsicum chinense Jacq. cv. Naga King Chili. Bioprocess and biosystems engineering, 2014, 37(6): 1055-1063.

Kehie, M., Kumaria, S., Tandon, P. Osmotic stress induced-capsaicin production in suspension cultures of Capsicum chinense Naga King Chili. Acta physiologiae plantarum, 2012, 34(5): 2039-2044.

Meyer, H.P., Schmidhalter, D. Industrial Scale Suspension Culture of Living Cells. John Wiley & Sons, Singapore, 2014, ISBN: 978-3-527-33547-3.

Ochoa-Alejo, N. Somatic Embryogenesis in Capsicum spp. In: Somatic Embryogenesis: Fundamental Aspects and Applications, Springer International Publishing, 2016, pp. 233-240, ISBN - 978-3-319-33704-3.

Omolo, M.A., Wong, Z.Z., Mergen, K., Hastings, J.C., Le, N.C., Reil, H.A., Case, K.A., Baumler, D.J. Antimicrobial properties of chili peppers. Journal of Infectious Diseases and Therapy, 2014, 2(4): 1000145.

Paek, K.Y., Murthy, H.N., Zhong, J.J. Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht, 2014, ISBN 978-94-017-9222-6.

Pavlov, A. I., Ilieva, M. P., Panchev, I. N. Nutrient medium optimization for rosmarinic acid production by Lavandulavera MM cell suspension. Biotechnology progress, 2000, 16(4): 668-670.

Pavlov, A., Georgiev, M., Bley, T. Batch and fed-batch production of betalains by red beet (Beta vulgaris) haroots in a bubble column reactor. ZeitschriftfürNaturforschung C, 2007, 62(5-6):439-446.

Reyes-Escogido, M.D.L., Gonzalez-Mondragon, E.G., Vazquez-Tzompantzi, E. Chemical and pharmacological aspects of capsaicin. Molecules, 2011, 16(2): 1253-1270.

Santana-Gálvez, J., Cisneros-Zevallos, L., Jacobo-Velázquez, D.A. Chlorogenic acid: Recent advances on its dual role as a food additive and a nutraceutical against metabolic syndrome. Molecules, 2017, 22(3), p.358.

Santos, M. R. A. D., Souza, C. A. D., Paz, E. S. Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño. Revista Ciência Agronômica, 2017, 48(3): 523-530.

Steingroewer J., Bley T., Georgiev V., Ivanov I., Lenk F., Marchev A., Pavlov A. Bioprocessing of differentiated plant in vitro systems. Engineering in Life Sciences. 2013, 13(1): 36-38.

How to Cite
SHEROVA, Gergana; PAVLOV, Atanas; GEORGIEV, Vasil. Polyphenols profiles and antioxidant activities of extracts from Capsicum chinense in vitro plants and callus cultures. Food Science and Applied Biotechnology, [S.l.], v. 2, n. 1, p. 30-37, mar. 2019. ISSN 2603-3380. Available at: <>. Date accessed: 18 may 2024. doi: