Magnetic nanoparticles based fluorescence immunoassay for food contaminants MNPs based immunoassay
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Abstract
Nanotechnology provides exciting new possibilities for advanced development of new analytical tools and instruments for bioanalytical applications. Magnetic nanoparticles (MNPs) have attracted much research interest in the past decade because they have good biocompatibility and can be readily separated from reaction mixtures with the aid of an external magnetic field. Heterogenic fluorescent immunoassays for determination of different analytes (antibiotics, pesticides, progesterone, aflatoxins, enterotoxins) using MNPs were developed. MNPs were prepared by thermal co-precipitation of Fe2+ and Fe3+ in aqueous solution. MNPs were functionalized with (3-aminopropyl) triethoxysilane. Two different analyzes were made for proving the successful modification of MNPs – FTIR and TEM. The corresponding antibodies were immobilized on modified MNPs. Then corresponding antigen - fluorescent dye conjugates were synthesized. The next step was purification of the resulting conjugates by gel filtration chromatography and proving them by UV-Vis and Fluorescence analyses. The 9 different separate competitive MNPs based immunoassays were developed – for penicillin, sulfonamide, tetracycline, progesterone, aflatoxin M1 and B1, enterotoxin A, paraoxon and dichlorvos. Then multiplex fluorescent immunoassay device based on magnetic nanoparticles for determination of penicillin, sulfonamide and tetracycline in milk sample was developed. The device ensured fast, selective and cheap antibiotic analysis. Very promising analytical characteristics for all three antibiotics were received - very low LODs and wide linear range. Practical applications: The multiplex fluorescent immunoassay device based on magnetic nanoparticles for determination of penicillin, sulfonamide and tetracycline in milk sample was developed. Device ensured fast, selective and cheap antibiotic analysis. Very promising analytical characteristics for all three antibiotics were received - very low LODs and wide linear range.
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References
Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemisty, 1976, 72: 248-254.
Chang, Y., Chen, D., Preparation adsorption properties of monodisperse chitosan bound Fe3O4 magnetic nanoparticles for removal of Cu (II) ions. Journal of Colloid and Interface Science, 2005, 283: 446-51. https://doi.org/10.1016/j.jcis.2004.09.010
Costa V.M., de Souza M.C.M., Fechine P.B.A., Macedo A.C., Gonçalves L.R.B., Nanobiocatalic systems based on lipase-Fe3O4 and conventional systems for isoniazid synthesis: A comparative study, Braz. J. Chem. Eng., 2016, 33 (3): 661. https://doi.org/10.1590/0104-6632.20160333s20150137
Cristea C., Tertis M., Galatus R. Magnetic Nanoparticles for Antibiotics Detection. Nanomaterials, 2017, 7(6): 119-125. https://doi.org/10.3390/nano7060119
Gabrovska K., Ivanova S., Ivanov Y., Godjevargova T. Immunofluorescent analysis with magnetic nanoparticles for simultaneous determination of antibiotic residues in milk. Analytical Letters, 2013, 46:1537–1552. https://doi.org/10.1080/00032719.2013.769264
Ivanova, T., Godjevargova, T. Sensitive progesterone determination using a magnetic particle based enzyme-linked immunosorbent assay. Analytical Letters, 2015, 48: 1-18. https://doi.org/10.1080/00032719.2014.963596
Ivanova S., Gabrovska K., Godjevargova T. Fluorescent immunoassay for determination of penicillin and sulphonamide residues in milk using carboxylic magnetic nanoparticles, International Journal of Dairy Technology, 2014, 67 (4):521-529.https://doi.org/10.1111/1471-0307.12172
Kirby M. S., Saad J. G., Immunoassays. Tools for Sensitive Specific, and Accurate Test Results, Laboratory medicine, 1996, 27 (3):177-193. https://doi.org/10.1093/labmed/27.3.177
Muratovic A. Z., Hagström T., Rosén J., Granelli K., Hellenäs K. E. Quantitative analysis of Staphylococcal enterotoxins A and B in food matrices using ultra high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Toxins, 2015, 7: 3637-3656.https://doi.org/10.3390/toxins7093637
Roque A C A, Bispo S, Pinheiro A R N, Antunes J M A, Goncalves D, and Ferreira H A. Antibody Immobilization on magnetic particles. Journal of Molecular recognition, 2009, 22: 77–82. https://doi.org/10.1002/jmr.913
Yaneva M., Ivanov Y., and Godjevargova T. Preparation of Polyclonal Antibodies with Application for an Organophosphorus Pesticide Immunoassay. Analytical letters, 2017, 50 (8): 1–18. https://doi.org/10.1080/00032719.2016.1221417
Yaneva M., Ivanov Y., Todorov N., Godjevargova T. Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk. Food and Agricultural Immunology,https://doi.org/10.1080/09540105.2017.1368458
Wang Y., Zou M., Yao P. and Xu Y. Multiplexing-Capable Immunobiosensor Based on Competitive Immunoassay, State of the Art in Biosensors - Environmental and Medical Applications, Dr. Toonika Rinken (Ed.), 2013.
Usleber E., Litz S., Märtlbauer E. Production and characterization of group‐specific and Agricultural Immunology, 1998, 10: 317-324. https://doi.org/10.1080/09540109809354995
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