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Nadezhda Petkova Manol Ognyanov Blaga Inyutin Petar Zhelev Panteley Denev


Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

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Ahmed A. E. R, Labavitch J. M. A simplified method for accurate determination of cell wall uronide content. Journal of Food Biochemistry, 1978, 1: 361-365

Aladedunye F., Matthäus B. Phenolic extracts from Sorbus aucuparia (L.) and Malus baccata (L.) berries: Antioxidant activity and performance in rapeseed oil during frying and storage, Food Chemistry, 2014, 159: 273-281

AOAC Official Method 920.39. Fat (crude) or ether extraction in animal feed. Gaithersburg, MD, USA, AOAC International, 2012

AOAC Official Method 934.06 for Moisture in Dried Fruits, Gaithersburg, MD, USA, AOAC International, 2007a

AOAC Official Method 940.26 Ash of Fruits and Fruit Products. Gaithersburg, MD, USA, AOAC International, 2007b

Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Analytical Biochemistry, 1973, 54: 484–489

Bradstreet R. B. The Kjeldahl method for organic nitrogen. Academic Press, New York, 1965, pp. 1-239. ISBN 13: 9780121246501

Dadwal V., Agrawal H., Sonkhla K., Joshi R., Gupta M. Characterization of phenolics, amino acids, fatty acids and antioxidant activity in pulp and seeds of high altitude Himalayan crab apple fruits (Malus baccata). Journal of Food Science and Technology, 2018, 55 (6): 2160-2169

Delgado-Pelayo R., Gallardo-Guerrero L., Hornero-Méndez D. Chlorophyll and carotenoid pigments in the peel and flesh of commercial apple fruit varieties. Food Research International, 2014, 65(B): 272-281

GB 5009.5-2010. National Food Safety Standard. Determination of protein in foods. National Standard of the People's Republic of China, People's Republic of China Ministry of Health issued 2010-03-26

Hallmann E., Orpel E., Rembiałkowska E. The content of biologically active compounds in some fruits from natural state. Vegetable Crops Research Bulletin, 2011, 75: 81-90

ISO 750:1998(E). Fruit and vegetable products - Determination of titratable acidity. Geneva, Switzerland: International Organization for Standardization (ISO), 1998

Ivanov I., Vrancheva R., Marchev A., Petkova N., Aneva I., Denev P, Georgiev V., Pavlov A. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. International Journal of Current Microbiology and Applied Sciences, 2014, 3(2): 296-306,%20et%20al.pdf

Kivrak I., Duru M., Öztürk M., Mercan N., Harmandar M., Topçu G. Antioxidant, anticholinesterase and antimicrobial constituents from the essential oil and ethanol extract of Salvia potentillifolia. Food Chemistry, 2009, 116 (2):470-479

Kumari A., Dhaliwal Y. S. A Study on nutritional composition and value addition of crab apple (Malus baccata). American Journal of Food Science and Technology, 2017, 5(1): 19-22

Kürschner K., Hoffer A. Eine neue quantitative cellulose bestimmung. Chemiker Zeitung. 1931, 55: 161-163.

Lee J., Durst R. W., Wrolstad R. E. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International, 2005, 88(5): 1269-1278

Li L., Su H., Ma H., Lyu D. Differential proteomic analysis reveals the effect of calcium on Malus baccata Borkh. leaves under temperature stress. International Journal of Molecular Sciences, 2017, 18 (8): 1755-1780

Li N., Shi J., Wang K. Profile and antioxidant activity of phenolic extracts from 10 crab apples (Malus wild species). Journal of Agriculture and Food Chemistry, 2014, 62: 574−581

Lichtenthaler H.K., Wellburn A.R., Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biochemical Society Transactions, 1983, 11(5): 591-592

Mezzomo N., Ferreira S. R. S. Carotenoids functionality, sources, and processing by supercritical technology: a review. Journal of Chemistry, 2016, Article ID 3164312, 1-16

Mulabagal V., van Nocker S., Dewitt D., Nair M. Cultivars of apple fruits that are not marketed with potential for anthocyanin production. Journal of Agriculture and Food Chemistry, 2007, 55(20): 8165–8169.

Padewska M. The importance of decorative trees and bushes for natural environment of Warsaw and estimation of nutritious value of selected fruits. Master Science thesis. Warsaw University of Life Science. 2008

Petkova N., Ivanov I., Denev P., Pavlov At. Bioactive substance and free radical scavenging activities of flour from jerusalem artichoke (Helianthus tuberosus L.) tubers – a comparative study. Turkish Journal of Agricultural and Natural Sciences, 2014a, Sp. 2:1773-1778

Petkova N., Vrancheva R., Denev P., Ivanov I., Pavlov A. HPLC-RID method for determination of inulin and fructooligosacharides. Acta Scientifica Naturalis, 2014b, 1: 99-107.

Petkova N., Petrova I., Ivanov I., Mihov R., Hadjikinova R., Ognyanov M., Nikolova V. Nutritional and antioxidant potential of carob (Ceratonia siliqua) flour and evaluation of functional properties of its polysaccharide fraction. Journal of Pharmaceutical Sciences and Research, 2017, 9: 2189–2195.

Petkova N., Ognyanov M. Phytochemical characteristics and in vitro antioxidant activity of fresh, dried and processed fruits of Cornelian cherries (Cornus mas L.). Bulgarian Chemical Communications, 2018, 50 (C): 302-307

Rudikovskaya E. G., Dudareva L. V., Shishparenok A. A., Mitanova N. B., Petrova I. G., Rudikovskii A. V. Phenolic composition of Malus baccata fruit. Chemistry of Natural Compounds, 2014, 50(4): 739-740.

Rudikovskaya E.G., Dudareva L. V., Shishparenok A. A., Rudikovskii A. V. Peculiarities of polyphenolic profile of fruits of Siberian crab apple and its hybrids with Malus domestica Borkh. Acta Physiologiae Plantarum, 2015, 238(37):1-8

Sharma R., Nath A. K., Antioxidant levels and activities of reactive oxygen-scavenging enzymes in crab apple fruits (Malus baccata), Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2016, 86(4): 877-885

Tsao R., Yang R., Yong J. C., Zhu H. Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC). Journal of Agriculture and Food Chemistry, 2003, 51(21): 6347-6353

Wang L., Wang Zh., Li X. Preliminary phytochemical and biological activities study of solvent extracts from a cold-field fruit—Malus baccata (Linn.) Borkh. Industrial Crops and Products, 2013, 47: 20-28.

Yoshizawa Y., Sakurai K., Kawaii S., Soejima J., Murofushi N. Antipoliferative and antioxidant activities of crab apple juices. Food Science and Technology Research, 2004, 10 (3): 278–281

How to Cite
PETKOVA, Nadezhda et al. Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits. Food Science and Applied Biotechnology, [S.l.], v. 3, n. 1, p. 47-55, mar. 2020. ISSN 2603-3380. Available at: <>. Date accessed: 07 apr. 2020. doi: