Optimization of polyphenols extraction process with antioxidant properties from wild Vaccinium myrtillus L. (bilberry) and Vaccinium vitis-idaea L. (lingonberry) leaves
Article Sidebar
Main Article Content
Abstract
The aim of the current study was to optimize the extraction condition of polyphenol compounds with antioxidant properties from leaves of natural grown Vaccinium myrtillus L and Vaccinium vitis-idaea L. The extractions were carried out in ultrasonic bath at 40 ºC for 20 minutes with different solvents (water, 20 % ethanol, 40 % ethanol, 60 % ethanol, 80 % ethanol and 96 % ethanol) and different hydro module of samples and solvents used (1:50, 1:100 and 1:200). The highest total phenol content in the leaves of V. myrtillus L. and V. vitis-idaea L. was found when 40% ethanol extract was used (90.50 ± 0.05 mg GAE/g DW and 96.68 ± 0.05 mg GAE/g DW, respectively). The highest total flavonoid content of the leaves of both species was obtained with 80 % ethanol as extraction solvent. The highest level of total proanthocyanidins were in the 60 % ethanol extract of V. myrtillus L. and in the 80 % ethanol extract of V. vitis-idaea L (13.12 ± 0.11 mg LE/g DW and 24.22 ± 0.21 mg LE/g DW, respectively). The highest ability to scavenge DPPH radicals possessed the 40 % ethanol extracts from the leaves of both species (693.99 ± 4.05 mM TE/g DW for V. myrtillus L. and 1083.18 ± 8.48 mM TE/g DW for V. vitis-idaea L). Data analysis showed that the maximal amount of polyphenols was extracted at a hydro-module of 1: 100. HPLC analysis revealed that the dominant phenolic acid in the leaves of V. myrtillus L was chlorogenic acid (13.45 mg/g DW), while ferulic acid (49.48 mg/g DW) was present at the highest concentration in the leaves of V. vitis-idaea L.
Article Details
References
Brasanac-Vukanovic S., Mutic J., Stankovic DM., Arsic I., Blagojevic N., Vukasinovic-Pesic V., Tadic VM. Wild bilberry (Vaccinium myrtillus L., Ericaceae) from Montenegro as a source of antioxidants for use in the production of nutraceuticals, Molecules, 2018, 23:1864. https://doi.org/10.3390/molecules23081864
Burdulis D., Sarkinas A., Jasutiené I., Stackevicené E., Nikolajevas L., Janulis V. Comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (Vaccinium myrtillus L.) and blueberry (vaccinium corymbosum L.) fruits Acta Poloniae Pharmaceutica – Drug Research. 2009, 66(4):399-408.
Chu W., Cheung SCM., Lau RAW., Benzie IFF. Chapter 4 Bilberry (Vaccinium myrtillus L.) NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. (IFF Benzie, S. Wachtel-Galor Eds.) Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor & Francis; 2011.
Claus T., Palombini SV., Carbonera F., Figueiredo IL., Matsushita M., Visentainer JV. Response surface methodology applied in the study of emulsion formulations in the presence of leaves of rosemary (Rosmarinus officinalis L.) as a source of natural antioxidants. Journal of the Brazilian Chemical Society, 2015, 26(10):2097-2104. http://dx.doi.org/10.5935/0103-5053.20150195
Cvetanović A., Švarc-Gajić J., Mašković P., Savić S., Nikolić LJ. Antioxidant and biological activity of chamomile extracts obtained by different techniques: perspective of using superheated water for isolation of biologically active compounds. Industrial Crops and Products, 2015, 65:582-591. https://doi.org/10.1016/j.indcrop.2014.09.044
Dincheva I., Badjakov I. Assessment of the anthocyanin variation in Bulgarian bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.). International Journal of Medicine and Pharmaceutical Science, 2016, 6(3), 39-50.
Hagerman, A., 2011. The Tannin Handbook Available At: http://www.users.muohio.edu/hagermae/tannin.pdf
Hokkanen J., Mattila S., Jaakola L., Pirttila AM., Tolonen A. Identification of phenolic compounds from lingonberry (Vaccinium vitis-idaea L.), bilberry (Vaccinium myrtillus L.) and hybrid bilberry (Vaccinium x intermedium Ruthe L.) leaves. Journal of Agricultural and Food Chemistry, 2009, 57, 9437–9447. https://doi.org/10.1021/jf9022542
Ivanov I., Vrancheva R., Marchev A., Petkova N., Aneva I., Denev P., Georgiev V., Pavlov A. Antioxidant activities and phenolic compounds in Bulgarian Fumaria species. International Journal of Current Microbiology and Applied Science, 2014, 3:296-306.
Mašković PZ., Diamanto LD., Cvetanović A., Radojković M., Spasojević MB., Zengin G. Optimization of the extraction process of antioxidants from orange using response surface methodology. Food Analytical Methods, 2016, 9:1436–1443. https://doi.org/10.1007/s12161-015-0321-5
Porter LJ., Hrstich LN., Chan BC. The conversion of urocyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry, 1985, 25(1):223-230. https://doi.org/10.1016/S0031-9422(00)94533-3
Radojković M., Zeković Z., Jokić S., Vidović S., Lepojević Z., Milošević S. Optimization of solid-liquid extraction of antioxidants from black mulberry leaves by response surface methodology. Food Technology and Biotechnology, 2012, 50(2):167–176.
Riihinen K., Jaakola L., Kärenlampi S., Hohtola A. Organ-specific distribution of phenolic compounds in bilberry (Vaccinium myrtillus) and ‘northblue’ blueberry (Vaccinium corymbosum x V. angustifolium). Food Chemistry, 2008, 110(1):156-160. https://doi.org/10.1016/j.foodchem.2008.01.057
Rohloff J., Uleberg E., Nes A., Krogstad T., Nestby R., Martinussen I. Nutritional composition of bilberries (Vaccinium myrtillus L.) from forest fields in Norway – Effects of geographic origin, climate, fertilization and soil properties. Journal of Applied Botany and Food Quality, 2015, 88:274-287. https://doi.org/10.5073/JABFQ.2015.088.040
Szakiel A., Pączkowski C., Huttunen S. Triterpenoid content of berries and leaves of bilberry Vaccinium myrtillus from Finland and Poland. Journal of Agricultural and Food Chemistry, 2012a, 60(48):11839-11849. https://doi.org/10.1021/jf3046895
Szakiel A., Paczowski C., Koivuniemi H., Huttunen S Comparison of the triterpenoids content of berries and leaves of lingonberry Vaccinium litis-idaea from Finland and Poland. Journal of Agricultural and Food Chemistry, 2012b, 60:4994-5002. https://doi.org/10.1021/jf300375b
Zeković Z., Cvetanović A., Pavlić B., Švarc-Gajić J., Radojković M. Optimization of the polyphenolics extraction from chamomile ligulate flowers using response surface methodology. International Journal of Plant Research, 2014, 4(2):43–50. https://doi.org/10.5923/j.plant.20140402.01
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Open access articles are distributed under the terms and conditions of the Creative Commons Attribution-Share Alike 4.0 International License (CC BY-SA 4.0) license:
https://creativecommons.org/licenses/by-sa/4.0
If you have any questions about the permitted uses of a specific article, please contact us.
Permissions Department of the Academic Publishing House of the UFT Plovdiv
Plovdiv 4002, 26 Maritsa Blvd., Bulgaria
E-mail: editor.in.chief@ijfsab.com
Tel.: +359 (32) 603-802
Fax: +359 32/ 644 102