Investigation of antimicrobial activity of polyphenol-enriched extracts against probiotic lactic acid bacteria
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The antimicrobial activity of polyphenol-enriched extracts from industrial plant by-products (strawberry and bilberry press residues and distilled rose petals) against probiotic lactic acid bacteria (Lactobacillus delbrueckii subsp. bulgaricus – S10 and S19; Lactobacillus rhamnosus – YW and S25; Lactobacillus gasseri – S20; Streptococcus thermophilus – S13 and S32) was investigated. The minimum inhibitory concentration (MIC) in most strains tested was found to be relatively high (from 6.25 mg.mL-1 to 12.50 mg.mL-1). The maximum concentration of polyphenols without inhibitory effect (MCWI) ranges from 0.390mg.mL-1 to 0.781mg.mL-1. The results obtained in the present study showed that among the tested lactic acid bacteria Lactobacillus delbrueckii subsp. bulgaricus – S19, Lactobacillus rhamnosus – YW and Streptococcus thermophilus – S13 had the best growth characteristics in polyphenol-enriched culture medium. These strains had the highest MIC and MCWI values and could be used as starter cultures for polyphenol-fortified fermented milks. Practical applications: The use of polyphenol-enriched extracts from industrial plant by-products (waste) – distilled rose petals (by-products of rose oil production) and strawberry and bilberry press residues (by-products of fruit juice production) contribute for improving the economic effect and for solving environmental problems in food industry. Development of functional fermented milks with combination of probiotic starter cultures and polyphenol extracts is current and perspective direction of food industry.
Cardona F., Andrés-Lacueva C., Tulipani S., Tinahones F.J, Queipo-Ortuño M.I. Benefits of polyphenols on gut microbiota and implications in human health. The Journal of Nutritional Biochemistry, 2013, 24(8): 1415-1422. https://doi.org/10.1016/j.jnutbio.2013.05.001
Cueva C., Moreno-Arribas M., Martín-Álvarez P.J., Bills G., Vicente M.F., Basilio A., Rivas C.L., Requena T., Rodríguez J.M., Bartolomé B. Antimicrobial activity of phenolic acids against commensal, probiotic and pathogenic bacteria. Research in Microbiology, 2010, 161(5): 372-382. https://doi.org/10.1016/j.resmic.2010.04.006
Daglia M. Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 2012, 23(2): 174-181. https://doi.org/10.1016/j.copbio.2011.08.007
Gyawali R., Ibrahim S.A. Impact of plant derivatives on the growth of foodborne pathogens and the functionality of probiotics. Applied Microbiology and Biotechnology, 2012, 95(1): 29-45. https://doi.org/10.1007/s00253-012-4117-x
Gyawali R., Ibrahim S.A. Natural products as antimicrobial agents. Food Control, 2014, 46(1): 412-429. https://doi.org/10.1016/j.foodcont.2014.05.047
Hervert-Hernández D., Goñi I. Dietary Polyphenols and Human Gut Microbiota: a Review. Food Reviews International, 2011, 27(2): 154-169. https://doi.org/10.1080/87559129.2010.535233
Manach C., Scalbert A., Morand C., Rémésy C., Jiménez L. Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition, 2004, 79(5): 727-747. https://doi.org/10.1093/ajcn/79.5.727
Marín L., Miguélez E.M., Villar C.J., Lombó F. Bioavailability of Dietary Polyphenols and Gut Microbiota Metabolism: Antimicrobial Properties. BioMed Research International, 2015, 2015: 18 pages. http://dx.doi.org/10.1155/2015/905215
Maukonen J., Saarela M. Human gut microbiota: does diet matter? Proceedings of the Nutrition Society, 2015, 74(1): 23-26. https://doi.org/10.1017/S0029665114000688
Pacheco-Ordaz R., Wall-Medrano A., Goñi1 M.G., Ramos-Clamont-Montfort G., Ayala-Zavala J.F., González-Aguilar G.A. Effect of phenolic compounds on the growth of selected probiotic and pathogenic bacteria. Applied Mi-crobiology, 2018, 66(1): 25-31. https://doi.org/10.1111/lam.12814
Scalbert A., Manach C., Morand C., Rémésy C., Jiménez L. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition, 2005, 45(4): 287-306. https://doi.org/10.1080/1040869059096
Tabasco R., Sánchez-Patán F., Monagas M., Bartolomé B., Moreno-Arribas M.V., Peláez C., Requena T. Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth: resistance and metabolism. Food Microbiology, 2011, 28(7): 1345-1352. https://doi.org/10.1016/j.fm.2011.06.005
Velderrain-Rodríguez G.R., Palafox-Carlos H., Wall-Medrano A., Ayala-Zavala J.F., Chen C.O., Robles-Sánchez M., Astiazaran-García H., Alvarez-Parrilla E., González-Aguilar G.A. Phenolic compounds: their journey after intake. Food Function, 2014, 5(2): 189-197. https://doi.org/10.1039/C3FO60361J
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