Royal jelly: chemical composition, health benefits and food applications: A review Royal jelly: chemical composition, health benefits and food applications
Main Article Content
Abstract
Royal jelly (RJ) is a valuable bee product with complex chemical composition, high nutritional value and numerous health benefits. This article aims to review the general chemical composition, health promoting properties and food applications of RJ. RJ has been found to be a biological product that is characterized by a substantial protein content (approximately 50% of its dry matter), followed by other essential nutrients and bioactive compounds such as carbohydrates, lipids, fatty acids, minerals, vitamins, enzymes, hormones and phenolic compounds (mainly flavonoids). Particular attention is paid to the biological properties and therapeutic effects of RJ such as antimicrobial, antiviral, antioxidant, anti-inflammatory, antitumor, anti-aging and many others. The medical indications, dosage and health benefits of RJ consumption, (recently hailed as a “superfood”) are also discussed. It can be concluded that RJ has attracted extensive research attention in recent years due to its great potential for application in the food industry as a functional food ingredient and in the manufacture of probiotic dairy products.
Article Details
References
Ahmad S., Campos M.G., Fratini F., Altaye S.Z., Li J. New Insights into the Biological and Pharmaceutical Properties of Royal Jelly. International Journal of Molecular Sciences, 2020, 21(2): 382. https://doi.org/10.3390/ijms21020382
Ahmadnia H., Sharifi N., Alizadeh S., Roohani Z., Kamalati A., Marjan S.S. Wonderful Effects of Royal Jelly on Treatment of Male-Factor Related Infertility. Austin Journal of Reproductive Medicine & Infertility, 2015, 2(6): 1031.
Ali A.M., Kunugi H. Royal jelly as an intelligent anti-aging agent—a focus on cognitive aging and alzheimer’s disease: a review. Antioxidants, 2020, 9(10): 937. https://doi.org/10.3390/antiox9100937
Alu'datt M.H., Rababah T., Obaidat M.M., Ereifej K., Alhamad M.N., Mhaidat N., Andrade J.E., Johargy A., Ayadi W. Probiotics in milk as functional food: characterization and nutraceutical properties of extracted phenolics and peptides from fermented skimmed milk inoculated with royal jelly. Journal of Food Safety, 2015, 35(4): 509–522. https://doi.org/10.1111/jfs.12201
Arzi A., Olapour S., Yaghooti H., Sistani K.N. Effect of royal jelly on formalin induced-inflammation in rat hind paw. Jundishapur Journal of Natural Pharmaceutical Products, 2015, 10(1): 22466.
https://doi.org/10.17795/jjnpp-22466
Aslan A., Cemek M., Buyukokuroglu M.E., Altunbas K., Bas O., Yurumez Y. Royal jelly can diminish secondary neuronal damage after experimental spinal cord injury in rabbits. Food and Chemical Toxicology, 2012, 50(7): 2554–2559. https://doi.org/10.1016/j.fct.2012.04.018
Asma S.T., Bobiş O., Bonta V. Acaroz U., Shah S.R.A., Istanbullugil F.R., Arslan-Acaroz D. General nutritional profile of bee products and their potential antiviral properties against mammalian viruses. Nutrients, 2022, 14(17): 3579. https://doi.org/10.3390/nu14173579
Atallah A.A. The production of bio-yoghurt with probiotic bacteria, royal jelly and bee pollen grains. Journal of Nutrition & Food Sciences, 2016, 6(3): 1000510. https://doi.org/10.4172/2155-9600.1000510
Bagameri L., Botezan S., Bobis O., Bonta V., Dezmirean D.S. Molecular insights into royal jelly anti-inflammatory properties and related diseases. Life, 2023, 13(7): 1573. https://doi.org/10.3390/life13071573
Bahari H., Taheri S., Rashidmayvan M., Hezaveh Z.S., Mousavi S. E., Malekahmadi M. The effects of Royal Jelly consumption on lipid profile: A GRADE-assessed systematic review and dose-response meta-analysis. Pharma Nutrition, 2023, 25(9): 100351. https://doi.org/10.1016/j.phanu.2023.100351
Bălan A., Moga M.A., Dima L., Toma S., Elena Neculau A., Anastasiu C.V. Royal Jelly - A traditional and natural remedy for postmenopausal symptoms and aging-related pathologies. Molecules, 2020, 25(14): 3291. https://doi.org/10.3390/molecules25143291
Balkanska R., Zhelyazkova I., Ignatova M. Physico-chemical quality characteristics of royal jelly from three regions of Bulgaria. Agricultural Science And Technology, 2012, 4(3): 302-305. Available at: https://agriscitech.eu/wp-content/uploads/2014/05/PQS_1-Physico-chemical-quality-characteristics-of-royal-jelly.pdf
Barnuiu L.I., Marghitas L.A., Dezmirean D.S., Mihai C.M., Bobis O. Chemical composition and antimicrobial activity of royal jelly - review. Scientific Papers: Animal Science and Biotechnologies, 2011, 44: 67–72. Print ISSN:1841-9364; ISSN: 2344-4576 (Online)
Bilikova K., Wub G., Simuth J. Isolation of a peptide fraction from honeybee Royal Jelly as a potential antifoulbrood factor. Apidologie, 2001, 32(3): 275-283. http://doi.org/10.1051/apido:2001129
Botezan S., Baci G.M., Bagameri L., Pasca C., Dezmirean D.S. Current status of the bioactive properties of royal jelly: a comprehensive review with a focus on its anticancer, anti-inflammatory, and antioxidant effects. Molecules, 2023, 28(3): 1510. https://doi.org/10.3390/molecules28031510
Buttstedt A., Ihling C.H., Pietzsch M. Moritz R.F.A. Royalactin is not a royal making of a queen. Nature, 2016, 537(9): E10. https://doi.org/10.1038/nature19349
Carpena M., Nuñez-Estevez B., Soria-Lopez A., Simal-Gandara J. Bee Venom: An updating review of its bioactive molecules and its health applications. Nutrients, 2020, 12(11): 3360. https://doi.org/10.3390/nu12113360
Chansuwan W., Khamhae M., Sirinupong N. Hydrolase-treated royal jelly attenuates LPS-induced inflammation and IgE-antigen-mediated allergic reaction. Functional Foods in Health and Disease, 2020, 10(3): 127. http://doi.org/10.31989/ffhd.v10i3.694
Chen Y.F., Wang K., Zhang Y.Z., Zheng Y.F.,. Hu F.L. In vitro anti-inflammatory effects of three fatty acids from royal jelly. Mediators of inflammation, 2016, 54(10). https://doi.org/10.1155/2016/3583684
Chen L., Deng H., Cui H., Fang J., Zuo Z., Deng J., Li Y., Wang X., Zhao L. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 2017, 14, 9(6): 7204-7218. https://doi.org/10.18632/oncotarget.23208
Chen Y.F., You M.M., Liu Y.C. Shi Y.Z., Wang K. Lu Y.Y., Hu F.L. Potential protective effect of trans-10-hydroxy-2-decenoic acid on the inflammation induced by lipoteichoic acid. Journal of Functional Foods, 2018, 45(6): 491-498. https://doi.org/10.1016/j.jff.2018.03.029
Cinar A., Altuntas S., Altuntas V. The addition of royal jelly to dairy probiotic dessert produced with predictive microbiology: Influence on physicochemical, rheological, microbial and sensorial properties. LWT – Food Science and Technology, 2021, 146(7): 111444. https://doi.org/10.1016/j.lwt.2021.111444
Collazo N., Carpena M., Nuñez-Estevez B., Otero P., Simal-Gandara J., Prieto M.A. Health promoting properties of bee royal jelly: food of the queens. Nutrients, 2021, 13(2): 543. https://doi.org/10.3390/nu13020543
Coutinho D., Karibasappa S.N., Mehta D.S. Royal jelly antimicrobial activity against periodontopathic bacteria. Journal of Interdisciplinary Dentistry, 2018, 8(1): 18-22. https://doi.org/10.4103/jid.jid_72_17
Darwish A.M.G., Abd El-Wahed A.A., Shehata M.G., El-Seedi H.R., Masry S.H.D., Khalifa S.A.M., Mahfouz H.M., El-Sohaimy S.A. Chemical profiling and nutritional evaluation of bee pollen, bee bread, and royal jelly and their role in functional fermented dairy products. Molecules, 2023, 28(1): 227. https://doi.org/10.3390/molecules28010227
El-Guendouz S., Lyoussi B., Miguel M.G. Insight into the chemical composition and biological properties of Mediterranean royal jelly. Journal of Apicultural Research, 2020, 59(5): 1–20. https://doi.org/10.1080/00218839.2020.1744241
El-Hanoun A., Elkomy A., Fares W., Shahien E. Impact of royal jelly to improve reproductive performance of male rabbits under hot summer conditions. World Rabbit Science, 2014, 22(3): 241-248. https://doi.org/10.4995/wrs.2014.1677
Flanjak I., Primorac L., Vukadin I., Kovačić M., Puškadija Z., Rajs B.B. Physicochemical characteristics of Croatian royal jelly. Croatian Journal of Food Science and Technology, 2019, 11(2): 266-271. https://doi.org/10.17508/CJFST.2019.11.2.18
Fratini F., Cillia G., Turci B., Feliciolli A. Beeswax: A minireview of its antimicrobial activity and its application in medicine. Asian Pacific Journal of Tropical Medicine, 2016a, 9(9): 839-843. https://doi.org/10.1016/j.apjtm.2016.07.003
Fratini F., Cilia G., Mancini S., Felicioli A. Royal Jelly: An ancient remedy with remarkable antibacterial properties. Microbiological Research, 2016b, 192(11): 130-141. https://doi.org/10.1016/j.micres.2016.06.007
Gao K., Su B., Dai J., Li P., Wang R., Yang X. Anti-Biofilm and Anti-Hemolysis Activities of 10-Hydroxy-2-decenoic Acid against Staphylococcus aureus. Molecules, 2022, 27(5): 1485. https://doi.org/10.3390/molecules27051485
Ghadimi-Garjan R., Javadi A., Jafarizadeh-Malmiri H., Anarjan N., Mirzaei H. Lyophilized royal jelly preparation in nanoscale and evaluation of its physicochemical properties and bactericidal activity. Food Science & Nutrition, 2023, 11(3): 3404–3413. https://doi.org/10.1002/fsn3.3330
Ghanbari E., Khazaei M.R., Khazaei M., Nejati V. Royal jelly promotes ovarian follicles growth and increases steroid hormones in immature rats. International Journal of Fertility and Sterility, 2018, 11(4): 263–269. https://doi.org/10.22074/ijfs.2018.5156
Granato D., Branco G.F., Cruz A.G., Faria J.A.F., Shah N.P. Probiotic dairy products as functional foods. Comprehensive Reviews in Food Science and Food Safety, 2010, 9(5): 455-470. https://doi.org/10.1111/j.1541-4337.2010.00120.x
Gu H., Song I.B., Han H.J., Lee N.Y., Cha J.Y., Son Y.K., Kwon J. Antioxidant activity of royal jelly hydrolysates obtained by enzymatic treatment. Korean Society for Food Science of Animal Resources, 2018, 38(1): 135-142. https://doi.org/10.5851/kosfa.2018.38.1.135
Guendouz M., Haddi A., Grar H., Kheroua O., Saidi D., Kaddouri H. Preventive effects of royal jelly against anaphylactic response in a murine model of cow's milk allergy. Pharmaceutical Biology, 2017, 55(1): 2145-2152. https://doi.org/10.1080/13880209
Guldas M. Effects of royal jelly and bee pollen on the growth of selected probiotic bacteria (Bf. animalis spp. lactis, L. acidophilus and L. casei). Journal of Apicultural Science, 2016, 60(2): 129-140. https://doi.org/10.1515/JAS-2016-0023
Guo H., Kouzuma Y., Yonekura M. Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 2009, 113(1): 238-245. https://doi.org/10.1016/j.foodchem.2008.06.081
Guo J., Wang Z., Chen Y., Cao Y, Tian W, Ma B., Dong Y. Active components and biological functions of royal jelly. Journal of Functional Foods, 2021, 82(7): 104514. https://doi.org/10.1016/j.jff.2021.104514
Habashy N.H., Abu-Serie M.M. Major royal-jelly protein 2 and its isoform X1 are two novel safe inhibitors for hepatitis C and B viral entry and replication. International Journal of Biological Macromolecules, 2019, 141(12): 1072-1087.
https://doi.org/10.1016/j.ijbiomac.2019.09.080
Habashy N.H., Abu-Serie M.M. The potential antiviral effect of major royal jelly protein2 and its isoform X1 against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): Insight on their sialidase activity and molecular docking. Journal of Functional Foods, 2020, 75(12): 104282. https://doi.org/10.1016/j.jff.2020.104282
Haddadin M.S.Y., Haddadin J., Benguiar R. The effect of royal jelly on growth and short-chain fatty acid production of probiotic bacteria and activity of bacterial procarcinogenic enzymes in rat faeces. Polish Journal of Food and Nutrition Sciences, 2012, 62(4): 251-258. https://doi.org/10.2478/v10222-012-0058-4
Hadi A., Najafgholizadeh A., Aydenlu E.S., Shafiei Z., Pirivand F., Golpour S., Pourmasoumi M. Royal jelly is an effective and relatively safe alternative approach to blood lipid modulation: A meta-analysis. Journal of Functional Foods, 2018, 41(2): 202-209. https://doi.org/10.1016/j.jff.2017.12.005
Han B., Li C., Zhang L., Fang Y., Feng M., Li J. Novel royal jelly proteins identified by gel-based and gel-free proteomics. Journal of Agricultural and Food Chemistry, 2011, 59(18): 10346–10355. https://doi.org/10.1021/jf202355n
Hashemipour M.A., Tavakolineghad Z., Arabzadeh S.A., Iranmanesh Z., Nassab S.A. Antiviral activities of honey, royal jelly, and acyclovir against HSV-1. Wounds, 2014, 26(2): 47-54. Available at: https://www.hmpgloballearningnetwork.com/site/wounds/article/antiviral-activities-honey-royal-jelly-and-acyclovir-against-hsv-1
Hassan A.A.M., Elenany Y.E., Nassrallah A., Cheng W., El-Maksoud A.A.A. Royal jelly improves the physicochemical properties and biological activities of fermented milk with enhanced probiotic viability. International journal of molecular sciences, 2022, 155(2): 112912. https://doi.org/10.1016/j.lwt.2021.112912
Husein M.Q., Kridli R.T. Reproductive responses following royal jelly treatment administered orally or intramuscularly into progesterone-treated Awassi ewes. Animal Reproduction Science, 2002, 74(1-2): 45-53. https://doi.org/10.1016/S0378-4320(02)00165-3
ISO 12824:2016(E). Royal jelly - specifications. Switzerland: International Organization for Standardization (ISO), 2016.
Kamakura M. Royalactin induces queen differentiation in honeybees. Nature, 2011, 473(7348): 478-483. https://doi.org/10.1038/nature10093
Karaca T., Şimşek N., Uslu S., Kalkan Y., Can I., Kara A., Yörük M. The effect of royal jelly on CD3+, CD5+, CD45+ T-cell and CD68+ cell distribution in the colon of rats with acetic acid-induced colitis. Allergologia et Immunopathologia, 2012, 40(6): 357-361. https://doi.org/10.1016/j.aller.2011.09.004
Kashima Y., Kanematsu S., Asai S., Kusada M., Watanabe S., Kawashima T., Nakamura T., Shimada M., Goto T., Nagaoka S. Identification of a novel hypocholesterolemic protein, major royal jelly protein 1, derived from royal jelly. PLOS One, 2014, 9(8): e105073. https://doi.org/10.1371/journal.pone.0105073
Kausar S.H., More V.R. Royal jelly: organoleptic characteristics and physicochemical properties. The Pharmaceutical and Chemical Journal, 2019, 6(2): 20-24. Available at: https://tpcj.org/download/vol-6-iss-2-2019/TPCJ2019-06-02-20-24.pdf
Kavas N. Functional probiotic yoghurt production with royal jelly fortification and determination of some properties. International Journal of Gastronomy and Food Science, 2022, 28(6): 100519. https://doi.org/10.1016/j.ijgfs.2022.100519
Khalifa H., Eleiwa N., Nazim H. Royal jelly, a super food, protects against celecoxib-induced renal toxicity in adult male albino. Canadian Journal of Kidney Health and Disease, 2024, 11(3):1-6. https://doi.org/10.1177/20543581241235526
Khazaei M., Ansarian A., Ghanbari E. New findings on biological actions and clinical applications of royal jelly: a review. Journal of Dietary Supplements, 2017,15(5): 1–19. https://doi.org/10.1080/19390211.2017.1363843
Kocot J., Kielczykowska M., Luchowska-Kocot D., Kurzepa J., Musik I. Antioxidant potential of propolis, bee pollen, and royal jelly: possible medical application. Oxidative Medicine and Cellular Longevity, 2018, 5: 7074209. https://doi.org/10.1155/2018/7074209
Kohguchi M., Inoue S.I., Ushio S., Iwaki K., Ikeda M., Kurimoto M. Effect of royal jelly diet on the testicular function of hamsters. Food Science and Technology Research, 2007, 10(4): 420–423. https://doi.org/10.3136/fstr.10.420
Kumar R., Thakur A., Kumar S., Hajam Y.A. Royal jelly a promising therapeutic intervention and functional food supplement: A systematic review. Heliyon, 2024, 10(17): e37138. https://doi.org/10.1016/j.heliyon.2024.e37138
Kunugi H., Ali A.M. Royal jelly and its components promote healthy aging and longevity: from animal models to humans. International Journal of Molecular Sciences, 2019, 20(19): 4662. https://doi.org/10.3390/ijms20194662
Lima W.G., Brito J.C.M., da Cruz Nizer W.S. Bee products as a source of promising therapeutic and chemoprophylaxis strategies against COVID-19 (SARS-CoV-2). Phytotherapy Research, 2020, 35(2): 6872. https://doi.org/10.1002/ptr.6872
Liu J.R., Yang Y.C., Shi L.S., Peng C.C. Antioxidant properties of royal jelly associated with larval age and time of harvest. Journal of Agricultural and Food Chemistry, 2008, 56(23): 11447-11452. https://doi.org/10.1021/jf802494e
Lyubenov L., Atanasov A., Hristakov I. Profitableness and perspective of the apiculture in North-Eastern Bulgaria. Conference: 27th Annual International Scientific Conference "Research for Rural Development 2021", Jelgava, Latvia, 2021, pp. 167.
https://doi.org/10.22616/rrd.27.2021.024
Maghsoudlou A., Mahoonak A.S., Mohebodini H., Toldra F. Royal Jelly: chemistry, storage and bioactivities. Journal of Apicultural Science, 2019, 63(6): 17-40. https://doi.org/10.2478/jas-2019-0007
Maleki V., Jafari-Vayghan H., Saleh-Ghadimi S., Adibian M., Kheirouri S., Alizadeh M. Effects of royal jelly on metabolic variables in diabetes mellitus: a systematic review. Complementary therapies in medicine, 2019, 43(4): 20-27. https://doi.org/10.1016/j.ctim.2018.12.022
Matsui T., Yukiyoshi A., Doi S., Sugimoto H., Yamada H., Matsumoto K. Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensive ability in SHR. The Journal of nutritional biochemistry, 2002, 13(2): 80-86. https://doi.org/10.1016/s0955-2863(01)00198-x
Mohamed A.A.R., Galal A.A., Elewa Y.H. Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain. Acta Histochemica, 2015, 117(7): 649-658. https://doi.org/10.1016/j.acthis.2015.07.002
Morita H., Ikeda T., Kajita K., Fujioka K., Mori I., Okada H., Uno Y., Ishizuka T. Effect of royal jelly ingestion for six months on healthy volunteers. Nutrition Journal, 2012, 11(9): 77. https://doi.org/10.1186/1475-2891-11-77
Moselhy W., Fawzy A., Kamel A. An evaluation of the potent antimicrobial effects and unsaponifiable matter analysis of the royal jelly. Life Science Journal, 2013, 10(2): 290-296. Available at: https://www.lifesciencesite.com/lsj/life1002/046_17060life1002_290_296.pdf
Mureşan C.I., Dezmirean D.S., Marc B.D., Suharoschi R., Pop O.L., Buttstedt A. Biological properties and activities of major royal jelly proteins and their derived peptides. Journal of Functional Foods, 2022, 98(11): 105286. https://doi.org/10.1016/j.jff.2022.105286
Nikolova T., Dimitrova I., Teneva A. The development of beekeeping in Bulgaria and the European Union in the last ten years. An overview. Bulgarian Journal of Animal Husbandry, 2023, 60(1): 37-45. Print ISSN: 2284-7995, e-ISSN: 2285-3952. Available at: https://animalscience-bg.org/page/bg/details.php?article_id=977
Oka H., Emori Y., Kobayashi N., Hayashi Y., Nomoto K. Suppression of allergic reactions by royal jelly in association with the restoration of macrophage function and the improvement of Th1/Th2 cell responses. International Immunopharmacology, 2001, 1(3): 521–532. http://doi.org/10.1016/s1567-5769(00)00007-2
Okumura N., Toda T., Ozawa Y., Watanabe K., Ikuta T., Tatefuji T., Hashimoto K., Shimizu T. Royal jelly delays motor functional impairment during aging in genetically heterogeneous male mice. Nutrients, 2018, 10(9): 1191. https://doi.org/10.3390/nu10091191
Oršolič N., Jembrek M.J. Royal jelly: biological action and health benefits. International Journal of Molecular Sciences, 2024, 25(11): 6023. https://doi.org/10.3390/ijms25116023
Osama H., Abdullah A., Gamal B., Emad D., Sayed D., Hussein E., Mahfouz E., Tharwat J., Sayed S., Medhat S., Bahaa T., Abdelrahim M.E.A. Effect of honey and royal jelly against cisplatin-induced nephrotoxicity in patients with cancer. Journal of the American College of Nutrition, 2017, 36(5): 342-346. http://doi.org/10.1080/07315724.2017.1292157
Pan Q., Pan H., Lou H., Xu Y., Tian L. Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo. Cancer Cell International, 2013, 13(7): 69. https://doi.org/10.1186/1475-2867-13-69
Pan Y., Xu J., Jin P., Yang Q., Zhu K., You M., Chen M., Hu F., Chen M. Royal jelly ameliorates behavioral deficits, cholinergic system deficiency, and autonomic nervous dysfunction in ovariectomized cholesterol-fed rabbits. Molecules, 2019, 24(6): 1149. https://doi.org/10.3390/molecules24061149
Pasupuleti V.R., Sammugam L., Ramesh N., Gan S.H. Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxidative Medicine and Cellular Longevity, 2017, Article ID: 1259510, pp. 1-21. https://doi.org/10.1155/2017/1259510
Polsinelli G.A., Yu H.D. Regulation of histone deacetylase 3 by metal cations and 10-hydroxy-2E-decenoic acid: Possible epigenetic mechanisms of queen-worker bee differentiation. PLOS ONE, 2018, 13(12): e0204538. https://doi.org/10.1371/journal.pone.0204538
Ramadan M.F., Al-Ghamdi A. Bioactive compounds and health-promoting properties of royal jelly: A review. Journal of Functional Foods, 2012, 4(1): 39-52. https://doi.org/10.1016/j.jff.2011.12.007
Ramanathan A.N.K.G., Nair A.J., Sugunan V.S. A review on royal jelly proteins and peptides. Journal of Functional Foods, 2018, 44(5): 255-264. https://doi.org/10.1016/j.jff.2018.03.008
Rizki A.M.F., Usman A.N., Raya I., Aliyah, Dirpan A., Arsyad A., Fendi F., Sumidarti A. Effect of royal jelly to deal with stress oxidative in preconception women: A literature review. Gaceta Sanitaria, 2021, 35(2): S288-S290. https://doi.org/10.1016/j.gaceta.2021.10.036
Roman Junior W.A., Piato A.L., Conterato G.M., Wildner S.M., Marcon M., Mocelin R., Emanuelli M.P., Emanuelli T., Nepel A., Barison A., Santos C.A.M. Hypolipidemic effects of Solidago chilensis hydroalcoholic extract and its major isolated constituent quercetrin in cholesterol-fed rats. Pharmaceutical Biology, 2015, 53(4): 1488-1495. https://doi.org/10.3109/13880209.2014.989622
Romanelli A., Moggio L., Montella R.C., Campiglia P., Iannaccone M., Capuano F., Pedone C., Capparelli R. Peptides from royal jelly: studies on the antimicrobial activity of jelleins, jelleins analogs and synergy with temporins. Jurnal of Peptides Science, 2011, 17(1): 348-352. https://doi.org/10.1002/psc.1316
Sabatini A.G., Marcazzan G.L., Caboni M.F., Bogdanov S., de Almeida-Muradian L.B. Quality and standardisation of Royal Jelly. Journal of ApiProduct and ApiMedical Science, 2009, 1(1): 1-6. https://doi.org/10.3896/IBRA.4.1.01.04
Salama S., Shou Q., Abd El-Wahed A.A., Elias N., Xiao J., Swillam A., Umair M., Guo Z., Daglia M., Wang K., Khalifa S.A.M., El-Seedi H.R. Royal jelly: beneficial properties and synergistic effects with chemotherapeutic drugs with particular emphasis in anticancer strategies. Nutrients, 2022, 14(19): 4166.
https://doi.org/10.3390/nu14194166
Sediva M., Laho M., Kohutova L., Mojzisova A., Majtan J., Klaudiny J.A. 10-HDA, a major fatty acid of royal jelly, exhibits ph dependent growth-inhibitory activity against different strains of Paenibacillus larvae. Molecules, 2018, 23(12): 3236. https://doi.org/10.3390/molecules23123236
Sesta G. Determination of sugars in royal jelly by HPLC. Apidologie, 2006, 37(1): 84-90. https://doi.org/10.1051/apido:2005061
Sesta G., Lusco L. Refractometric determination of water content in royal jelly. Apidologie, 2008, 39(2): 225–232.
https://doi.org/10.1051/apido:2007053
Sharif S.N., Darsareh F. Effect of royal jelly on menopausal symptoms: A randomized placebo-controlled clinical trial. Complementary Therapies in Clinical Practice, 2019, 37(11): 47-50. https://doi.org/10.1016/j.ctcp.2019.08.006
Spanidi E., Athanasopoulou S., Liakopoulou A., Chaidou A., Hatziantoniou S., Gardikis K. Royal jelly components encapsulation in a controlled release system - skin functionality, and biochemical activity for skin applications. Pharmaceuticals, 2022, 15(8): 907. https://doi.org/10.3390/ph15080907
Strant M., Yücel B., Topal E., Puscasu A.M., Margaoan R., Varadi A. Use of royal jelly as functional food in human and animal health. Journal of Animal Production, 2019, 60(2): 131-144. https://doi.org/10.29185/hayuretim.513449
Taavoni S., Barkhordari F., Goushegir A., Haghani H. Effect of royal jelly on premenstrual syndrome among iranian medical sciences students: a randomized, triple-blind, placebo-controlled study. Complementary Therapies in Medicine, 2014, 22(4): 601-606. https://doi.org/10.1016/j.ctim.2014.05.004
Tahir R.A., Bashir A., Yousaf M.N., Ahmed A., Dali Y., Khan S., Sehgal S.A. In Silico identification of angiotensin-converting enzyme inhibitory peptides from MRJP1. PLOS ONE, 2020, 15(2): e0228265. https://doi.org/10.1371/journal.pone.0228265
Takaki-Doi S., Hashimoto K., Yamamura M., Kamei C. Antihypertensive activities of royal jelly protein hydrolysate and its fractions in spontaneously hypertensive rats. Okayama University Medical School, 2009, 63: 57-64. https://doi.org/10.18926/AMO/31859
Tamura S., Amano S., Kono T., Kondoh J., Yamaguchi K., Kobayashi S., Ayabe T., Moriyama T. Molecular characteristics and physiological functions of major royal jelly protein 1 oligomer. Proteomics, 2009, 9(24): 5534-5543. https://doi.org/10.1002/pmic.200900541
Tian W., Li M., Guo H., Peng W., Xue X., Hu Y., Liu Y., Zhao Y., Fang X., Wang K., Li X., Tong Y., Conlon M.A., Wu W., Ren F., Chen Z. Architecture of the native major royal jelly protein 1 oligomer. Nature Communications, 2018, 9(1): 3373. https://doi.org/10.1038/s41467-018-05619-1
Ulubayram N., Cinar A. Microencapsulated and fresh royal jelly: monitoring 10-hda content, antibacterial and antifungal activity at different storage periods. Brazilian Archives of Biology and Technology, 2023, 66: e23220203. https://doi.org/10.1590/1678-4324-2023220203
Uversky V.N., Albar A.H., Khan R.H., Redwan E.M. Multifunctionality and intrinsic disorder of royal jelly proteome. Proteomics, 2021, 21(1): 2000237. https://doi.org/10.1002/pmic.202000237
Wang S.Y., Chen C.W. Effects of royal jelly extracts on growth inhibition, differentiation human leukemic U937 cells and its immunomodulatory activity. Biocell, 2019, 43(1-1): 29-41.
Williams M.J., Sottoriva A., Graham T.A. Measuring clonal evolution in cancer with genomics. Annual review of genomics and human genetics, 2019, 20(5). https://doi.org/10.1146/annurev-genom-083117-021712
Wytrychowski M., Chenavas S., Daniele G., Casabianca H., Batteau M., Guibert S., Brion B., Physicochemical characterisation of french royal jelly: comparison with commercial royal jellies and royal jellies produced through artificial bee-feeding. Journal of Food Composition and Analysis, 2013, 29(2): 126–133. https://doi.org/10.1016/j.jfca.2012.12.002
Yang Y.C., Chou W.M., Widowati D.A., Lin I.P., Peng C.C. 10-hydroxy-2-decenoic acid of royal jelly exhibits bactericide and anti-inflammatory activity in human colon cancer cells. BMC Complementary Medicine and Therapies, 2018, 18(7): 202. https://doi.org/10.1186/s12906-018-2267-9
Yükünç G.O. Royal jelly: proteins and peptides. Journal of Apitherapy and Nature, 2019, 2(2): 59-70. https://doi.org/10.35206/jan.679534
Zhang S., Shao Q., Geng H., Su S. The effect of royal jelly on the growth of breast cancer in mice. Oncology letters, 2017, 14(7): 7615-7621. https://doi.org/10.3892/ol.2017.7078
Zheng J., Lai W., Zhu G., Wan M., Chen J., Tai Y., Lu C. 10-Hydroxy-2-decenoic acid prevents ultraviolet A-induced damage and matrix metalloproteinases expression in human dermal fibroblasts. Journal of the European Academy of Dermatology and Venereology, 2013, 27(10): 1269–1277.
https://doi.org/10.1111/j.1468-3083.2012.04707.x

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Open access articles are distributed under the terms and conditions of the Creative Commons Attribution-Share Alike 4.0 International License (CC BY-SA 4.0) license:
https://creativecommons.org/licenses/by-sa/4.0
If you have any questions about the permitted uses of a specific article, please contact us.
Permissions Department of the Academic Publishing House of the UFT Plovdiv
Plovdiv 4002, 26 Maritsa Blvd., Bulgaria
E-mail: editor.in.chief@ijfsab.com
Tel.: +359 (32) 603-802
Fax: +359 32/ 644 102