Main Article Content

Ivan Georgiev Ivanov Lydia Georgieva-Krusteva Vasil Georgiev Atanas Pavlov Radka Vrancheva


We report for the first time fumaric acid biosynthesis by in vitro systems of Fumaria spp. The highest amount of fumaric acid (137 mg/L) was accumulated by Fumaria officinalis L. plant cell culture, cultivated at submerged conditions in darkness. The prospects for efficient process development were also discussed.

Article Details


Das R., Brar S., Varma M. Recent advances in the biomedical applications of fumaric acid and its ester derivatives: The multifaceted alternative therapeutics. Pharmacological Reports, 2016, 68(2): 404-414.

Georgieva L., Ivanov I., Marchev A., Aneva I., Denev P.,Georgiev V., Pavlov A. Protopine production by Fumaria cell suspension cultures: Effect of light. Applied Biochemistry and Biotechnology, 2015, 176(1): 287–300.

He C-L., Fu B-D., Shen H-Q., Jiang X-L., Wei X-B. Fumaric acid, an antibacterial component of Aloe vera L. African Journal of Biotechnology, 2011, 10(15): 2973–2977.

Ivanov I., Georgiev V., Pavlov A. Elicitation of galanthamine biosynthesis by Leucojum aestivum liquid shoot cultures. Journal of Plant Physiology, 2013, 170(12): 1122–1129.

Jowkar F., Jamshidzadeh A., MirzadehYazdi A., Pasalar M.The effects of Fumaria parviflora L. extract on chronichand eczema: a randomized doubleblind placebocontrolled clinical trial. Iranian Red Crescent Medical Journal, 2011,13(11): 824-828.

Kreuter A., Gambichler T., Altmeyer P., Brockmeyer N. Treatment of disseminated granuloma annular with fumaric acid esters. BMC Dermatology, 2002, 2: 5

Kuroda K., Akao M. Antitumor and anti-intoxication activities of fumaric acid in cultured cells. GAN. GANN; The Japanese Journal of cancer research, 1981, 72(5): 777–782.

Moharregh-Khiabani D., Linker RA., Gold R., Stangel M. Fumaric acid and its esters: An emerging treatment for multiple sclerosis. Current Neuropharmacology, 2009, 7: 60–64.

Pavlov A., Georgiev M., Panchev I., Ilieva M. Optimization of rosmarinic acid production by Lavandula vera MM plant cell suspension in a laboratory bioreactor. Biotechnology Progress, 2005, 21(2): 394–396.

Roa Engel C., Straathof A., Zijlmans T., van Gulik W., van der Wielen L. Fumaric acid production by fermentation. Applied Microbiologyand Biotechnology, 2008, 78: 379–389.

Schweckendiek W. Treatment of psoriasis vulgaris. Medizinische Monatsschrift für Pharmazeuten Journal, 1959, 13: 103–104.

Shakya A., Singh G., Chatterjee S., Kumar V. Role of fumaric acid in antiinflammatory and analgesic activities of a Fumaria indica extracts. Journal of Intercultural Ethnopharmacology, 2014, 3(4): 173–178.

Steingroewer J., Bley T., Georgiev V., Ivanov I., Lenk F., Marchev A., Pavlov A. Bioprocessing of differentiated plant in vitro systems. Engineering in Life Sciences, 2013, 13(1): 26–38.

Vrancheva R., Ivanov I., Aneva I., Dincheva I., Badjakov I, Pavlov A. Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria. Zeitschrift fuer Naturforschung C, 2016, 71(1-2): 9-14.

The Scientific Committee for Animal Nutrition (SCAN). Report of the scientific committee on animal nutrition on the safety of fumaric acid. European Commission Health and Consumer Protection Directorate General,

How to Cite
IVANOV, Ivan Georgiev et al. Production of fumaric acid from Fumaria sp. plant in vitro systems. Food Science and Applied Biotechnology, [S.l.], v. 2, n. 1, p. 62-66, mar. 2019. ISSN 2603-3380. Available at: <>. Date accessed: 25 may 2019. doi: