Main Article Content

Nguyen Thi Thao Ta Thac Binh Pham Thi Khanh Linh Vu Hong Son Nguyen Thi Minh Tu


Spirulina is a spiral cyanobacteria with simple cell structure, making it easier to extract chlorophyll than other materials. Investigation the ultrasound assisted extraction of chlorophyll a was conducted on the following factors: type of solvent, concentration of solvent, ratio of solvent: material, time and temperature. Optimization by Design Expert the extraction of chlorophyll a from spirulina algae indicates the condition to obtain the highest amount of chlorophyll a of 15.07 mg/g was as followings: solvent ethanol concentration 99.7%, ratio solvent / material: 16/1, time 2.5 hours, temperature 52°C, ultrasonic frequency 40kHz.

Article Details


Barrett J., Jeffrey S.W. Chlorophyllase and formation of an atypical chlorophyllide in marine algae. Plant Physiology, 1964, 39(1): 44-47.

Cohen Z. The chemicals of spirulina, In: Spirulina Platensis Arthrospira. Physiology, Cell-Biology and Biotechnology (A.E. Vonshak Ed.). CRC Press. 1997, pp. 175-204, ISBN: 9780748406746, eBook ISBN: 9780429079948,

Challouf R., Trabelsi L., Dhieb R.B., Abed O., Yahia A., Ghozzi K., Ammar J.B., Omran H., Ouada H.B. Evaluation of cytotoxicity and biological activities in extracellular polysaccharides released by cyanobacterium arthrospira platensis. Brazilian Archives of Biology and Technology, 2011, 54(4): 831-838. Available at:

Choi, W.Y., Lee, H.Y. Enhancement of chlorophyll a production from marine spirulina maxima by an optimized ultrasonic extraction process. Applied Sciences, 2018, 8(1): 8010026.

Hosikian A., Lim S., Halim R., Danquah M.K. Chlorophyll extraction from microalgae: A review on the process engineering aspects. International Journal of Chemical Engineering 2010, 2010(6): 391632.

Minchev I., Petkova N., Milkova-Tomova I. Ultrasound-assisted extraction of chlorophylls and phycocyanin from Spirulina platensis, Biointerface Research in Applied Chemistry, 2021, 11(2): 9296-9304.

Karsten U., Schumann R., Haubner N., Klausch S., Chlorophyll extraction methods for the quantification of green microalgae colonizing building facades. International Biodeterioration & Biodegradation, 2005, 55(3): 213-222.

Kong W., Liu N., Zhang J., Yang Q., Hua S., Song H., Xia C. Optimization of ultrasound-assisted extraction parameters of chlorophyll from Chlorella vulgaris residue after lipid separation using response surface methodology. Journal of Food Science and Technology, 2014, 51(9): 2006-2013.

Munawaroh H.S.H., Fathur M.R., Gumilar G., Aisyah S., Yuliani G., Mudzakir A., Wulandari A.P. Characterization and physicochemical properties of chlorophyll extract from Spirulina sp. Journal of Physics: Conference Series, 2019, 1280(2): 022013.

Macías-Sánchez M.D., Mantell C., Rodríguez M., Martínez de la Ossa E., Lubián L.M., Montero O., Comparison of supercritical fluid and ultrasound-assisted extraction of carotenoids and chlorophyll a from Dunaliella salina. Talanta, 2009, 77(3): 948-952.

Sánchez M., Bernal-Castillo J., Rozo C., Rodríguez I. Spirulina (arthrospira): An edible microorganism: A review. Universitas Scientiarum.Revista de la Facultad de Sciencias. 2003, 8(1): 7-24. Available at:,5

Phang S.M., Miah M.S., Yeoh B.G., Hashim M.A. Spirulina cultivation in digested sago starch factory wastewater. Journal of Applied Phycology, 2000, 12(3): 395-400.

Rahim A., Çakir C., Ozturk M., Şahin B., Soulaimani A., Sibaoueih M., Nasser B., Eddoha R., Essamadi A., El Amiri B. Chemical characterization and nutritional value of Spirulina platensis cultivated in natural conditions of Chichaoua region (Morocco). South African Journal of Botany, 2021, 141(9): 235-242.

Rosa G.M., Moraes L., Cardias B.B, de Souza M.R.A.Z., Costa J.A.V. Chemical absorption and CO2 biofixation via the cultivation of Spirulina in semicontinuous mode with nutrient recycle. Bioresource Technology, 2015, 192(9): 321-327.

Schumann R., Häubner N., Klausch S., Karsten U. Chlorophyll extraction methods for the quantification of green microalgae colonizing building facades. International Biodeterioration & Biodegradation, 2005, 55(3): 213-222.

Simon D., Helliwell S. Extraction and quantification of chlorophyll a from freshwater green algae. Water Research, 1998, 32(7): 2220-2223.

Tavanandi H.A., Raghavarao K.S.M.S. Recovery of chlorophylls from spent biomass of Arthrospira platensis obtained after extraction of phycobiliproteins. Bioresource Technology, 2019, 271(1): 391-401.

Wasmund N., Topp I., Schories D. Optimising the storage and extraction of chlorophyll samples. Oceanologia, 2006, 48(1): 125-144. Available at:

How to Cite
THAO, Nguyen Thi et al. Optimization of the extraction conditions for Chlorophyll a from fresh Spirulina. Food Science and Applied Biotechnology, [S.l.], v. 5, n. 1, p. 99-105, mar. 2022. ISSN 2603-3380. Available at: <>. Date accessed: 22 may 2022. doi: