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This research investigated the effects of postharvest storage on cyanide content and rhodanese isolated from bitter cassava (Manihot Utilissima) tubers. Cyanide content of freshly harvested sample, samples stored for 4 days and samples stored for 8 days were estimated by silver nitrate titration method. Rhodanese was purified using 80% ammonium sulphate precipitate and ion-exchange chromatography on CM-Sephadex C-25.
Cyanide content from freshly harvested sample was 345.6 mg HCN/kg while 237.6 mg HCN/kg and 108 mg HCN/kg were obtained for samples stored for 4 days and samples stored for 8 days respectively. Specific activity of rhodanese from freshly harvested sample was 3.411 RU/mg while 5.92 RU/mg and 5.35 RU/mg were obtained for samples stored for 4 days and samples stored for 8 days respectively. The Km values of rhodanese for KCN were 3.18 mM, 2.40 mM, 0.25 mM for freshly harvested sample, samples stored for 4 days and samples stored for 8 days respectively. The optimum temperature from freshly harvested sample and sample stored for 4 days was 70 0C while samples stored for 8 days was 50 0C. An optimum pH of 4.0 was obtained from the 3 samples.
Rhodanese play plausible role in cyanide reduction during the postharvest storage.
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