Tobacco, tobacco by-products and waste valorization opportunities Tobacco, tobacco by-products and waste valorization opportunities
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Abstract
Tobacco holds huge potential as a source of bioactive compounds, edible proteins, terpenoids, antioxidants and other active components beyond the mass production of cigarettes and other smoking products. Tobacco production and processing generates a large amount of waste and by-products, such as flower buds and seeds, stems, stalks, and leaves. Waste like cigarette butts has significant role in the environment pollution globally. Implementing sustainable and circular economy-oriented methods to the whole chain of tobacco life (harvesting – processing – usage – waste) is crucial to minimize litter and pollution and to fully valorize the plant. The value of tobacco is high in the pharmaceutical industry due to its chemical components. Tobacco is the richest source of solanesol, which is a key intermediate in coenzyme Q10 and vitamin K2 synthesis. Derived compounds of tobacco include alkaloids (anabasine, anatabine, nicotine, nicotine derivatives), phenolic compounds (chlorogenic acid, scopoletin, rutin), cellulose, and pectin. The phytoremediation feature of tobacco should be highlighted as a powerful method for soil detoxification. Furthermore, tobacco could be used for natural pesticides and biocontrol agents’ production. Many studies point out opportunities for bioenergy produced by tobacco waste. Recycling cigarette butts is researched and worked on. Challenges include toxicity management.
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Abdellah Y., Yang S., Yang C., Yang Z., Elsheikh E.A.E., Keiblinger K.M., Mohamed T.A., Rana M.S., Liu D., Yu F. Tobacco waste valorization through composting: A systematic review of biomass conversion efficiency and circular bioeconomy strategies. Biomass and Bioenergy, 2025, 201(10): 108137. https://doi.org/10.1016/j.biombioe.2025.108137
Akhtar N., Mannan M.A.U. Mycoremediation: expunging environmental pollutants. Biotechnology Reports, 2020, 26(6): e00452. https://doi.org/10.1016/j.btre.2020.e00452
Aldeek F., Lopez V., Miller J.H. Salting-out-assisted liquid-liquid extraction method for the determination of nicotine from oral traditional and innovative tobacco products using UPLC-MS/MS. ACS Omega, 2023, 8(34): 31256-31264. https://doi.org/10.1021/acsomega.3c03474
Alhowail A. Molecular insights into the benefits of nicotine on memory and cognition. Molecular Medicine Reports, 2021, 23(6): 398-403. https://doi.org/10.3892/mmr.2021.12037
Antika L.D., Tasfiyati A.N., Hikmat H., Septama A.W. Scopoletin: a review of its source, biosynthesis, methods of extraction, and pharmacological activities. Zeitschrift für Naturforschung C, 2022, 77(7-8)c: 303-316. https://doi.org/10.1515/znc-2021-0193
Arat S.A. A review on cigarette butts: Environmental abundance, characterization, and toxic pollutants released into water from cigarette butts. Science of the Total Environment, 2024, 928(6): 172327. https://doi.org/10.1016/j.scitotenv.2024.172327
Banožić M., Aladić K., Jerković I., Jokić S. Volatile organic compounds of tobacco leaves versus waste (scrap, dust, and midrib): extraction and optimization. Journal of the Science of Food and Agriculture, 2021, 101(5): 1822-1832. https://doi.org/10.1002/jsfa.10796
Banožić M., Babić J., Jokić S. Recent advances in extraction of bioactive compounds from tobacco industrial waste-a review. Industrial Crops and Products, 2020, 144(2): 112009. https://doi.org/10.1016/j.indcrop.2019.112009
Bareschino P., Marrasso E., Roselli C. Tobacco stalks as a sustainable energy source in civil sector: Assessment of techno-economic and environmental potential. Renewable Energy, 2021, 175(9): 373-390. https://doi.org/10.1016/j.renene.2021.04.101
Beulens J.W., Booth S.L., van den Heuvel E.G., Stoecklin E., Baka A., Vermeer C. The role of menaquinones (vitamin K₂) in human health. British Journal of Nutrition, 2013, 110(8): 1357-1368. https://doi.org/10.1017/S0007114513001013
Beutel M.W., Harmon T.C., Novotny T.E., Mock J., Gilmore M.E., Hart S.C., Traina S., Duttagupta S., Brooks A., Jerde C.L., Hoh E., Van De Werfhorst L.C., Butsic V., Wartenberg A.C., Holden P.A. A review of environmental pollution from the use and disposal of cigarettes and electronic cigarettes: contaminants, sources, and impacts. Sustainability, 2021, 13(23): 12994. https://doi.org/10.3390/su132312994
Bi R., Schlaak M., Siefert E., Lord R., Connolly H. Influence of electrical fields (AC and DC) on phytoremediation of metal polluted soils with rapeseed (Brassica napus) and tobacco (Nicotiana tabacum). Chemosphere, 2011, 83(3): 318-326. https://doi.org/10.1016/j.chemosphere.2010.12.052
Boffey D. Swedish firm deploys crows to pick up cigarette butts. The Guardian. 2022. Available at: https://www.theguardian.com/environment/2022/feb/01/swedish-crows-pick-up-cigarette-butts-litter
Bonanomi G., Maisto G., De Marco A., Cesarano G., Zotti M., Mazzei P., Libralato G., Staropoli A., Siciliano A., De Filippis F., La Storia A., Piccolo A., Vinale F., Crasto A., Guida M., Ercolini D., Incerti G. The fate of cigarette butts in different environments: Decay rate, chemical changes and ecotoxicity revealed by a 5-years decomposition experiment. Environmental Pollution, 2020, 261(6): 114108. https://doi.org/10.1016/j.envpol.2020.114108
Bosco F., Mollea C. Mycoremediation in soil. In: Environmental Chemistry and Recent Pollution Control Approaches (H. Saldarriaga-Noreña, M.A. Murillo-Tovar, R. Farooq, R. Dongre, S. Riaz Eds.). InTech Open, London, UK. 2019, pp. 173-188, Print ISBN 978-1-83968-062-5, eBook (PDF) ISBN 978-1-83968-064-9. https://doi.org/10.5772/intechopen.80247
Bribi N. Pharmacological activity of alkaloids: A Review. Asian Journal of Botany, 2018, 1(2): 1-6. https://doi.org/10.63019/ajb.v1i2.467
Chaney R.L., Baklanov I.A. Phytoremediation and Phytomining: Status and Promise. In: Advances in Botanical Research (A. Cuypers, J. Vangronsveld Eds.). Academic Press, Elsevier. 2017, 83, pp. 189-221, Print ISBN 9780123815187. https://doi.org/10.1016/bs.abr.2016.12.006
Cheynier V., Comte G., Davies K., Lattanzio V., Martens S. Plant phenolics: Recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiology and Biochemistry, 2013, 72: 1-20. https://doi.org/10.1016/j.plaphy.2013.05.009
Da Silva R.P.F.F., Rocha-Santos T.A.P., Duarte A.C. Supercritical fluid extraction of bioactive compounds. TrAC Trends in Analytical Chemistry, 2016, 76(2): 40-51. https://doi.org/10.1016/j.trac.2015.11.013
Dave B., Moysa E.L., Kuźnik, A. Nicotine degradation by Trametes versicolor: Insights from diverse environmental stressors and wastewater medium. Molecules, 2025, 30(12): 2658. https://doi.org/10.3390/molecules30122658
De Fenzo A., Giordano M., Sansone L. A clean process for obtaining high-quality cellulose acetate from cigarette butts. Materials (Basel), 2020, 13(21): 4710. https://doi.org/10.3390/ma13214710
Dehaghi E., Saed S., Tahami S., Solatifar N., Taghipoor M. Using waste cigarette butt fibers as asphalt binder stabilizer in stone matrix asphalt: mechanical and environmental characterization. Journal of Materials in Civil Engineering, 2024, 36(7). https://doi.org/10.1061/JMCEE7.MTENG-17251
Dewey R.E., Xie J. Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum. Phytochemistry, 2013, 94: 10-27. https://doi.org/10.1016/j.phytochem.2013.06.002
Ding Z., Dai Y., Hao H., Pan R., Yao X., Wang Z. Anti-inflammatory effects of scopoletin and underlying mechanisms. Pharmaceutical Biology, 2009, 46(12): 854-860. https://doi.org/10.1080/13880200802367155
Dussan K., Girisuta B., Haverty D., Leahy J.J., Hayes M.H.B. Kinetics of levulinic acid and furfural production from Miscanthus giganteus. Bioresource Technology, 2013, 149(12): 216-224. https://doi.org/10.1016/j.biortech.2013.09.006
Escobar V., Gozalo G., Pérez C. Variability and performance study of the sound absorption of used cigarette butts. Materials, 2019, 12(16): 2584. https://doi.org/10.3390/ma12162584
Escobar V., González C., Gozalo G. Analysis of the influence of thickness and density on acoustic absorption of materials made from used cigarette butts. Materials, 2021, 14(16): 4524. https://doi.org/10.3390/ma14164524
Esentürk-Güzel İ., Yapar Е., Sindhu R., Kaur H., Kara B. Tobacco: Its conventional and modern dosage forms in medication. Bezmialem Science, 2022, 10(5): 655-665. https://doi.org/10.14235/bas.galenos.2021.6492
Fratiglioni L., Wang H.X. Smoking and Parkinson’s and Alzheimer’s disease: review of the epidemiological studies. Behavioural Brain Research, 2000, 113(1): 117-120. https://doi.org/10.1016/S0166-4328(00\00206-0)
Frutos M.J., Rincón-Frutos L., Valero-Cases E. Rutin. In: Nonvitamin and Nonmineral Nutritional Supplements (S.M. Nabavi, A.S. Silva Eds.). Academic Press, Elsevier. 2019, pp. 111-117, ISBN: 978-0-12-812491-8. https://doi.org/10.1016/B978-0-12-812491-8.00015-1
Ganeshpurkar A., Saluja A.K. The pharmacological potential of rutin. Saudi Pharmaceutical Journal, 2017, 25(2): 149-164. https://doi.org/10.1016/j.jsps.2016.04.025
Gao X.Y., Li X.Y., Zhang C.Y., Bai C.Y. Scopoletin: a review of its pharmacology, pharmacokinetics, and toxicity. Frontiers in Pharmacology, 2024, 15(2): 1268464. https://doi.org/10.3389/fphar.2024.1268464
Geist H.J. Tobacco and deforestation revisited. How to move towards a global land-use transition? Sustainability, 2021, 13(16): 9242. https://doi.org/10.3390/su13169242
Global tobacco market size available at (access on December 25 2025) https://www.imarcgroup.com/tobacco-processing-plant
Grebenstein P.E., Erickson P., Grace M., Kotz C.M. Anatabine, nornicotine, and anabasine reduce weight gain and body fat through decreases in food intake and increases in physical activity. Journal of Clinical Medicine, 2022, 11(3): 481. https://doi.org/10.3390/jcm11030481
Green D.S., Tongue A.D.W., Boots B., The ecological impacts of discarded cigarette butts. Trends in Ecology & Evolution, 2022, 37(2): 183-192. https://doi.org/10.1016/j.tree.2021.10.001
Guan Y., Shi H., Xu T., Zhao Y., Meng X., Liu J. Extraction and purification of nicotine from tobacco waste by a sustainable aqueous two-phase system containing recyclable hydrophilic alcohol and salt. ACS Sustainable Resource Management, 2025, 2(6): 907-914. https://doi.org/10.1021/acssusresmgt.4c00416
Gui Z.Q., Yuan X.L., Yang J., Du Y.M., Zhang, P. An updated review on chemical constituents from Nicotiana tabacum L.: Chemical diversity and pharmacological properties. Industrial Crops and Products, 2024, 214(8): 118497. https://doi.org/10.1016/j.indcrop.2024.118497
Gullón B., Lú-Chau T.A., Moreira M.T., Lema J.M., Eibes G. Rutin: a review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends in Food Science & Technology, 2017, 67(9): 220-235. https://doi.org/10.1016/J.TIFS.2017.07.008
Guo X., Li Y., Liu C., Fu Y., Gao N. Anatabine: a substitute for the medicinal activity of nicotine. European Journal of Pharmacology, 2026, 178537. https://doi.org/10.1016/j.ejphar.2026.178537
Guo Y., Hiatt E., Bonnet C., Kudithipudi C., Lewis R., Shi H., Patra B., Zhao X., de Borne F., Gilles T., Yang S., Zhang H., Zhang M., Lusso M., Berger I., Xu D., Wen L. Chapter 5 - Molecular regulation and genetic manipulation of alkaloid accumulation in tobacco plants. Studies in Natural Products Chemistry, 2021, 70: 119-149. https://doi.org/10.1016/B978-0-12-819489-8.00006-5
Hossain M., Abdulla F., Rahman A., Huq M. N. Cigarette waste: A burden to the health, environment, and economy. Ecotoxicology and Environmental Safety, 2022, 239(7): 113661. https://doi.org/10.1016/j.ecoenv.2022.113661
Hristozova G.Y., Zaprjanova P.S., Marinova S.G. Relationships between soil characteristics and concentrations of lead and zinc in soils and in oriental tobacco. Ecologia Balkanica, 2020, 12(1): 1-10. Available at: http://web.uni-plovdiv.bg/mollov/EB/2020_vol12_iss1/001-010_eb.19151.pdf
Hu R.S., Wang J., Li H., Ni H., Chen Y.F., Zhang Y.W., Xiang S.P., Li H.H. Simultaneous extraction of nicotine and solanesol from waste tobacco materials by the column chromatographic extraction method and their separation and purification. Separation and Purification Technology, 2015, 146(5): 1-7. https://doi.org/10.1016/j.seppur.2015.03.016
Ignat I., Volf I., Popa V. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry, 2011, 126(4): 1821-1835. https://doi.org/10.1016/j.foodchem.2010.12.026
Ilkaeva M., Krivtsov I., García-López E.I., Marcì G., Khainakova O., García J.R., Palmisano L., Díaz E., Ordóñez S. Selective photocatalytic oxidation of 5-hydroxymethylfurfural to 2, 5-furandicarboxaldehyde by polymeric carbon nitride-hydrogen peroxide adduct. Journal of Catalysis, 2018, 359(3): 212-222. https://doi.org/10.1016/j.jcat.2018.01.012
Ikhsanov Y.S., Nauryzbaev M., Musabekova A., Alimzhanova M., Burashev E. Study of Nicotiana tabacum L extraction, by methods of liquid and supercritical fluid extraction. Journal of Applied Engineering Science, 2019, 17(3): 338-353. https://doi.org/10.5937/jaes17-21189
Kadir A., Mohajerani A. Leachability of heavy metals from fired clay bricks incorporated with cigarette butts. In: Papers Presented to 8th International Symposium on Lowland Technology. Bandung, Indonesia, 2012, p. 872-877. Available at: https://ieeexplore.ieee.org/document/6423017
Kakoria A., Sinha-Ray S. Ultrafine nanofiber-based high efficiency air filter from waste cigarette butts. Polymer, 2022, 255(8): 125121. https://doi.org/10.1016/j.polymer.2022.125121
Kameshwar, A.K.S., Qin W. Comparative study of genome-wide plant biomass-degrading CAZymes in white rot, brown rot and soft rot fungi. Mycology, 2018, 9(2): 93-105. https://doi.org/10.1080/21501203.2017.1419296
Kaminski K., Bovet L., Hilfiker A., Laparra H., Schwaar J., Sierro N., Lang G., De Palo D., Guy P., Laszlo C., Goepfert S., Ivanov N. Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.). BMC Genomics, 2023, 24(1): 516-528. https://doi.org/10.1186/s12864-023-09588-8
Khan I.U., Qi S.S., Gul F., Manan S., Rono J.K., Naz M., Shi X.N., Zhang H., Dai Z.C., Du D.L. A green approach used for heavy metals ‘phytoremediation’ via invasive plant species to mitigate environmental pollution: a review. Plants, 2023, 12(4): 725. https://doi.org/10.3390/PLANTS12040725
Kheawfu K., Kaewpinta A., Chanmahasathien W, Rachtanapun P, Jantrawut P. Extraction of nicotine from tobacco leaves and development of fast dissolving nicotine extract film. Membranes (Basel), 2021, 11(6): 403. https://doi.org/10.3390/membranes11060403
Kozak K, Antosiewicz D.M. Tobacco as an efficient metal accumulator. Biometals, 2023, 36(2): 351-370. https://doi.org/10.1007/s10534-022-00431-3
Kukula-Koch W.A., Widelski J. Alkaloids. In: Pharmacognosy: Fundamentals, Applications and Strategies (S. Badal, R. Delgoda Eds.). Academic Press Elsevier. 2017, pp. 163-198. ISBN: 978-0-12-802104-0, https://doi.org/10.1016/B978-0-12-802104-0.00009-3
Kumari P., Kumar V., Kumar R., Pahuja S. Sorghum polyphenols: plant stress, human health benefits, and industrial applications. Planta, 2021, 254(3): 47. https://doi.org/10.1007/s00425-021-03697-y
Kurmus H., Mohajerani A. The toxicity and valorization options of cigarette butts. Waste Management, 2020, 104(3): 104-118. https://doi.org/10.1016/j.wasman.2020.01.011
Kurmus H., Mohajerani A., Grist S. Polycyclic aromatic hydrocarbons (PAHs) in fired clay bricks incorporating cigarette butts. Materials (Basel), 2021, 14(8): 2032. https://doi.org/10.3390/ma14082032
Lakhan S., Kirchgessner A. Anti-inflammatory effects of nicotine in obesity and ulcerative colitis. Journal of Translational Medicine, 2011, 9(129): 1-10. https://doi.org/10.1186/1479-5876-9-129
Lazarova K., Christova D., Karashanova D., Georgieva B., Marovska G., Slavov A., Babeva T. Blending approach preparation of PVA-g-PMA films with embedded “green” synthesized silver nanoparticles for acetone optical detection. Sensors, 2023, 23(6): 2941. https://doi.org/10.3390/s23062941
Lee B.J., Huang Y.C., Chen S.J., Lin P.T. Coenzyme Q10 supplementation reduces oxidative stress and increases antioxidant enzyme activity in patients with coronary artery disease. Nutrition, 2012, 28(3): 250-255. https://doi.org/10.1016/j.nut.2011.06.004
Li Y., Chen J.P., Duan L., Li S. Effect of vitamin K2 on type 2 diabetes mellitus: A review. Diabetes Research and Clinical Practice, 2018, 136(2): 39-51. https://doi.org/10.1016/j.diabres.2017.11.020
Li Z.-J., Yang D.D., Wei Z.-Y., Huang J., Chi Y.-Q., Lu Y.-X., Yin F.-W. Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications. Biotechnology for Biofuels and Bioproducts, 2024, 17(11): 144. https://doi.org/10.1186/s13068-024-02593-3
Lin X., Li Q., Pu M., Dong H., Zhang Q. Significance of nicotine and nicotinic acetylcholine receptors in Parkinson's disease. Frontiers in Aging Neuroscience, 2025, 17(3): 1535310. https://doi.org/10.3389/fnagi.2025.1535310
Liu Y., Lin M., Mu X., Qin L., Deng J., Liu Y., Wu X., He W., Pang H., Han F., Sun C., Nie X. Protective effect of solanesol in glucose-induced hepatocyte injury: Mechanistic insights on oxidative stress and mitochondrial preservation. Chemico-Biological Interactions, 2023, 383(10): 110676. https://doi.org/10.1016/j.cbi.2023.110676
Liu J., Ma G., Chen T., Hou Y., Yang S., Zhang K.-Q., Yang J. Nicotine-degrading microorganisms and their potential applications. Applied Microbiology and Biotechnology, 2015, 99(5): 3775-3785. https://doi.org/10.1007/s00253-015-6525-1
Lu P., Wu R., Xu G., Zhang B. Effective separation of nicotine from tobacco extract by silica gel. Chemical Engineering Research and Design, 2025, 217(9): 502-513. https://doi.org/10.1016/j.cherd.2025.04.030
Ludwiczuk A., Skalicka-Woźniak K., Georgiev M.I. Alkaloids. In: Pharmacognosy: Fundamentals, Applications and Strategies (S. Badal, R. Delgoda Eds.). Academic Press Elsevier. 2017, pp. 233-266. ISBN: 978-0-12-802104-0, https://doi.org/10.1016/B978-0-12-802104-0.00011-1
Luo Y., Yang Y., Schneider C., Balle T. The аnti-nociceptive effects of nicotine in humans: A systematic review and meta-analysis. Pharmaceuticals, 2023, 16(12): 1665. https://doi.org/10.3390/ph16121665
Maderuelo Sanz R., Gómez Escobar V., Meneses Rodríguez J. Potential use of cigarette filters as sound porous absorber. Applied Acoustics, 2018, 129(1): 86-91. https://doi.org/10.1016/j.apacoust.2017.07.011
Mandić N., Lalević B., Raičević V., Radojičić V. Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste. International Journal of Environmental Science and Technology, 2023, 20(7): 7787-7798. https://doi.org/10.1007/s13762-022-04405-3
Manthos G., Tsigkou K. Upscaling of tobacco processing waste management: A review and a proposed valorization approach towards the biorefinery concept. Journal of Environmental Management, 2025, 387(8): 125942. https://doi.org/10.1016/j.jenvman.2025.125942
Marinello S., Lolli F., Gamberini R., Rimini B. A second life for cigarette butts? A review of recycling solutions. Journal of Hazardous Materials, 2019, 384(2): 121245. https://doi.org/10.1016/j.jhazmat.2019.121245
Martín C., Fernández T., García R., Carrillo E., Marcet M., Galbe M., Jönsson L.J. Preparation of hydrolysates from tobacco stalks and ethanolic fermentation by Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology, 2002, 18(9): 857-862. https://doi.org/10.1023/A:1021258708507
Massadeh R., El-Elimat T., Al-Gharaibeh M., Tawaha K., Alali F. UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca. Acta Pharmaceutica, 2022, 72(1): 97-108. https://doi.org/10.2478/acph-2022-0001
Meng X., Wang X., Guan Y. Salting-out-assisted liquid-liquid extraction for nicotine from its aqueous solutions. Journal of Chemical & Engineering Data, 2022, 67(2): 453-462. https://doi.org/10.1021/acs.jced.1c00916
Missaoui B., Krafft J.M., Hamdi N., Saliba V., BenJemaa J.M., Boujday S., Bergaoui L. Valorizing industrial tobacco wastes within natural clays and chitosan nanocomposites for an ecofriendly insecticide. Waste Management, 2023, 168(8): 146-155. https://doi.org/10.1016/j.wasman.2023.05.051
Mohajerani A., Tanriverdi Y., Nguyen B., Wong K., Dissanayake H., Johnson L., Whitfield D., Thomson G., Alqattan E., Rezaei A. Physico-mechanical properties of asphalt concrete incorporated with encapsulated cigarette butts. Construction and Building Materials, 2017, 153(10): 69-80. https://doi.org/10.1016/j.conbuildmat.2017.07.091
Morales-Vera R., Cantillana J., Arto-Paz F., Hernández C., Echeverría-Vega A., Valdés C. Treatment of cigarette butts: Biodegradation of cellulose acetate by rot fungi and bacteria. Microorganisms, 2024, 12(11): 2285. https://doi.org/10.3390/microorganisms12112285
Morone A., Apte M., Pandey R.A. Levulinic acid production from renewable waste resources: Bottlenecks, potential remedies, advancements and applications. Renewable and Sustainable Energy Reviews, 2015, 51(11): 548-565. https://doi.org/10.1016/j.rser.2015.06.032
Musk A.W., De Klerk N.H. History of tobacco and health. Respirology, 2003, 8(3): 286-290. https://doi.org/10.1046/j.1440-1843.2003.00483.x
Nagargade M., Singh P., Kumar S. Commercial Crops Other than Vegetables for a Green Economy of India. In: A Critical Appraisal of India’s Self-Reliance in Agriculture (P.K. Rai, N.K. Sharma, V. K. Singh, A.K Rai Eds). Nova Science Publishers, Inc. 2023, pp. 103-131. Print ISBN: 979-8-88697-602-1. https://doi.org/10.52305/FTUU7256
Nahar N., Rahman A., Nawani N.N., Ghosh S., Mandal A. Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis Thaliana. Journal of Plant Physiology, 2017, 218(11): 121-126. https://doi.org/10.1016/j.jplph.2017.08.001
Naveed M., Hejazi V., Abbas M., Kamboh A.A., Khan G.J., Shumzaid M., Ahmad F., Babazadeh D., FangFang X., Modarresi-Ghazani F., WenHua L., XiaoHui Z. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomedicine & Pharmacotherapy, 2018, 97(1): 67-74. https://doi.org/10.1016/j.biopha.2017.10.064
Ncube E., Mhlongo M., Piater L., Steenkamp P., Dubery I., Madala N. Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chemistry Central Journal, 2014, 8(66): 1-10. https://doi.org/10.1186/s13065-014-0066-z
Nenov V. Investigation of the techno-technological parameters during production of tobacco foil through extrusion and rolling. PhD thesis by University of Food Technologies of Bulgaria, 2007 [in Bulgarian]
Newhouse P.A., Kelton M. Nicotinic systems in central nervous systems disease: degenerative disorders and beyond. Pharmacochemistry Library, 2000, 31: 91-101. https://doi.org/10.1016/S0165-7208(00\80005-7)
Nguyen B.T., Dinh D.H., Hoang N.B., Do T.T., Milham P., Hoang D.T., Cao S.T. Composted tobacco waste increases the yield and organoleptic quality of leaf mustard. Agrosystems, Geosciences & Environment, 2022, 5(3): e20283. https://doi.org/10.1002/agg2.20283
Novotny T. E., Slaughter, E. Tobacco product waste: An environmental approach to reduce tobacco consumption. Current Environmental Health Reports, 2014, 1(3): 208-216. https://doi.org/10.1007/s40572-014-0016-x
Paulista С., Ferreira E., Guimarães C., Delaqua G., Lopera H., da Silva M., Vieira C. A new proposal to reduce microplastics from cigarette butts: Production of eco-friendly fired clay bricks. Construction and Building Materials, 2024, 449(11): 138514. https://doi.org/10.1016/j.conbuildmat.2024.138514
Peševski M.D., Iliev, B.M., Živković, D.L., Popovska, V.T.J., Srbinoska, M.A., Filiposki, B.K. Possibilities for utilization of tobacco stems for production of energetic briquettes. Journal of Agricultural Sciences, 2010, 55(1): 45-54. https://doi.org/10.2298/JAS1001045P
Núñez M.P., Butler M., Majul L.M., Diorio L.A., Levin L.N., Rosenfeldt S. Colonization of cigarette butts, a toxic waste, by white rot fungi. Revista Argentina de Microbiología, 2023, 55(3): 289-290. https://doi.org/10.1016/j.ram.2023.01.003
Popova V., Ivanova T., Stoyanova A., Georgiev V., Hristeva T., Nikolova V., Docheva M., Nikolov N., Damyanova S. Phytochemicals in leaves and extracts of the variety “Plovdiv 7” of Bulgarian oriental tobacco (Nicotiana tabacum L.). Trends in Phytochemical Research, 2018, 2(1): 27-36.
Prasad K., Pathak D., Patel A., Dalwadi P., Prasad R., Patel P., Selvaraj K. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology, 2011, 10(41): 8122-8130. https://doi.org/10.5897/AJB11.394
Prokopov C., Popova V., Nikolova M., Dimov M., Ivanova T., Taneva D. Equilibrium study of Cr (VI) removal from aqueous solution by stalks from three tobacco species (Nicotiana) grown in Bulgaria. Environmental Research, Engineering and Management, 2019, 75(3): 46-54. https://doi.org/10.5755/j01.erem.75.3.23343
Puripattanavong J., Songkram C., Lomlim L., Amnuaikit T. Development of concentrated emulsion containing Nicotiana tabacum extract for use as pesticide. Journal of Applied Pharmaceutical Science, 2013, 3(11): 16-21. https://doi.org/10.7324/JAPS.2013.31104
Rahman T., Mohajerani A. Use of bitumen encapsulated cigarette butts in stone mastic asphalt. Construction and Building Materials, 2020, 261(10): 120530. https://doi.org/10.1016/j.conbuildmat.2020.120530
Rahman T., Mohajerani A., Giustozzi F. Possible recycling of cigarette butts as fiber modifier in bitumen for asphalt concrete. Materials, 2020, 13(3): 734. https://doi.org/10.3390/ma13030734
Rajesh R.O., Godan T.K., Rai A.K., Sahoo D., Pandey A., Binod P. Biosynthesis of 2, 5-furan dicarboxylic acid by Aspergillus flavus APLS-1: Process optimization and intermediate product analysis. Bioresource Technology, 2019, 284: 155-160. https://doi.org/10.1016/j.biortech.2019.03.105
Ribeiro E., Teixeira M., Gatti T., Andrade R., Suarez P. Catalytic biorefining of cigarette butts recycling waste. Chemistry, 2025, 7(3): 86. https://doi.org/10.3390/chemistry7030086
Rodgman A., Perfetti T.A. The chemical components of tobacco and tobacco smoke (Second Edition). CRC Press, Boca Raton, FL, USA, 2013, eBook ISBN: 9780429096266. https://doi.org/10.1201/b13973
Ru Q., Wang L., Li W., Wang J., Ding Y. In vitro antioxidant properties of flavonoids and polysaccharides extract from tobacco (Nicotiana tabacum L.) leaves. Molecules, 2012, 17(9): 11281-11291. https://doi.org/10.3390/molecules170911281
Ruan A., Liu C. Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting. Water Science and Engineering, 2015, 8(4): 309-314. https://doi.org/10.1016/j.wse.2015.11.003
Rubio L.C.H. Estrategias para la degradación de colillas de cigarillo. PhD thesis by Pontificia Universidad Javeriana of Colombia, 2020 [in Spanish].
Shen K., Xia L., Gao X., Li C., Sun P., Liu Y., Fan H., Li X., Han L., Lu C., Jiao K., Xia C., Wang Z., Deng B., Pan F., Sun T. Tobacco as bioenergy and medical plant for biofuels and bioproduction. Heliyon, 2024, 10(13): e33920. https://doi.org/10.1016/j.heliyon.2024.e33920
Shah G., Bhatt U., Soni V. A comprehensive review on triple R eco-management strategies to reduce, reuse and recycle of hazardous cigarette butts. Heliyon, 2023, 9(6): e16642. https://doi.org/10.1016/j.heliyon.2023.e16642
Selwal N., Tabassum Z., Rahayu F., Yulia N.D., Sugiono S., Endarto O., Riajaya P.D., Djajadi D., Khamidah A., Wani A.K. Therapeutic potential and phytoremediation capabilities of the tobacco plant: Advancements through genetic engineering and cultivation techniques. Biocatalysis and Agricultural Biotechnology, 2023, 52(11): 102845. https://doi.org/10.1016/j.bcab.2023.102845
Shoji T., Hashimoto T., Saito K. Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids. Journal of Experimental Botany, 2024, 75(6): 1741-1753. https://doi.org/10.1093/jxb/erad341
Shults C.W., Oakes D., Kieburtz K., Beal M.F., Haas R., Plumb S., Juncos J.L., Nutt J., Shoulson I., Carter J., Kompoliti K., Perlmutter J.S., Reich S., Stern M., Watts R.L., Kurlan R., Molho E., Harrison M., Lew M., and the Parkinson Study Group (2002). Effects of coenzyme Q10 in early Parkinson disease: Evidence of slowing of the functional decline. Archives of Neurology, 2002, 59(10): 1541-1550. https://doi.org/10.1001/archneur.59.10.1541
Singh S., Kaur I., Kariyat R. The multifunctional roles of polyphenols in plant-herbivore interactions. International Journal of Molecular Sciences, 2021, 22(3): 1442. https://doi.org/10.3390/ijms22031442
Soleimani F., Dobaradaran S., Vazirizadeh A., Mohebbi G., Ramavandi B., De-la-Torre G., Nabipour I., Schmidt T., Novotny T., Maryamabadi A., Kordrostami Z. Chemical contents and toxicity of cigarette butts leachates in aquatic environment: A case study from the Persian Gulf region. Chemosphere, 2023, 311(2): 137049. https://doi.org/10.1016/j.chemosphere.2022.137049
Sophanodorn K., Unpaprom Y., Whangchai K., Homdoung N., Dussadee N., Ramaraj R. Environmental management and valorization of cultivated tobacco stalks by combined pretreatment for potential bioethanol production. Biomass Conversion and Biorefinery, 2022, 12(4): 1627-1637. https://doi.org/10.1007/s13399-020-00992-8
Steppuhn A., Gase K., Krock B., Halitschke R., Baldwin I.T. Nicotine’s defensive function in nature. PLOS Biology, 2004, 2(8): 1074-1080. https://doi.org/10.1371/journal.pbio.0020217
Su Y., Xian H., Shi S., Zhang C., Manik S.M.N., Mao J., Zhang G., Liao W., Wang Q., Liu H. Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk. BMC Biotechnology, 2016, 16(11): 81. https://doi.org/10.1186/s12896-016-0311-8
Suharto T., Sudirman, Ulfa M., Ningsih B., Putri K., Thamsiri P. Effect of water-methanol binary solvent system in the green synthesis of copper nanoparticles with derived from tobacco leaf extract. Acta Chimica Asiana, 2024, 7: 390-395. Available at: https://aca.unram.ac.id/index.php/ACA/article/view/197
Taylor M.A., Fraser P.D. Solanesol: Added value from Solanaceous waste. Phytochemistry, 2011, 72(11): 1323-1327. https://doi.org/10.1016/j.phytochem.2011.03.015
Teixeira M.B.H., Duarte M.A.B., Raposo Garcez L., Camargo Rubim J., Hofmann Gatti T., Suarez P.A.Z. Process development for cigarette butts recycling into cellulose pulp. Waste Management, 2017, 60(2): 140-150. https://doi.org/10.1016/j.wasman.2016.10.013
Torras-Claveria L., Jáuregui O., Codina C., Tiburcio A., Bastida J., Viladomat F. Analysis of phenolics compounds by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry in senescent and water-stressed tobacco. Plant Science, 2012, 182(1): 71-78. https://doi.org/10.1016/j.plantsci.2011.02.009
Tuzzin G., Godinho M., Dettmer A., Zattera A.J. Nanofibrillated cellulose from tobacco industry wastes. Carbohydrate Polymers, 2016, 148(9): 69-77. https://doi.org/10.1016/j.carbpol.2016.04.045
Vera-Marmanillo V., Churata R., Ortiz-Valdivia Y., Gordillo-Alarcón C., Tupayachy-Quispe D., Almirón J. Mineralizing effectiveness of Pleurotus ostreatus and Pleurotus djamor in the treatment of lignocellulosic waste from cigarette butts, Cusco (Peru). Revista De Gestão Social E Ambiental - RGSA, 2024, 18(12): e09851. https://doi.org/10.24857/rgsa.v18n12-083
Wang J., Lu D., Zhao H., Jiang B., Wang J., Ling X., Chai H., Ouyang P. Discrimination and classification of tobacco wastes by identification and quantification of polyphenols with LC-MS/MS. Journal of the Serbian Chemical Society, 2010, 75(7): 875-891. https://doi.org/10.2298/JSC091109055W
Wang J., Xu K., Zhang J., Ren G., Yang X., Zhang Z., Zhang Y., Xiao Y., Du Y. Systematic activity-oriented separation and structure-activity relationship of tobacco cembranoids. Industrial Crops and Products, 2021, 173(12): 114136. https://doi.org/10.1016/j.indcrop.2021.114136
Wang W., Yao J., Cao X. Alkaloids from tobacco leaves: Isolation, alkaloid contents, and potential application as a natural pesticide against Bactrocera dorsalis. BioResources, 2022, 17(1): 1764-1780. https://doi.org/10.15376/biores.17.1.1764-1780
Wang Y., Luo X., Chu P., Shi H., Wang R., Li J., Zheng S. Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil. Bioresources and Bioprocessing, 2023a, 10(2): 10. https://doi.org/10.1186/s40643-023-00630-x
Wang Z., Ma L., Liu P., Luo Z., Li Z., Wu M., Xu X., Pu W., Huang P., Yang J. Transcription factor NtWRKY33a modulates the biosynthesis of polyphenols by targeting NtMYB4 and NtHCT genes in tobacco. Plant Science, 2023b, 326: 111522. https://doi.org/10.1016/j.plantsci.2022.111522
Wu X., Singh S.K., Patra B., Wang J., Pattanaik S., Yuan L. An overview of the regulation of specialized metabolism in tobacco. Current Plant Biology, 2025, 41(3): 100431. https://doi.org/10.1016/j.cpb.2024.100431
Wu Z., Yang J., Zhang Y., Wang C., Guo S., Yu Y. Growth responses, accumulation, translocation and distribution of vanadium in tobacco and its potential in phytoremediation. Ecotoxicology and Environmental Safety, 2021, 207(1): 111297. https://doi.org/10.1016/j.ecoenv.2020.111297
Xiang G., Yang H., Yang L., Zhang X., Cao Q., Miao M. Multivariate statistical analysis of tobacco of different origin, grade and variety according to polyphenols and organic acids. Microchemical Journal, 2010, 95(2): 198-206. https://doi.org/10.1016/j.microc.2009.12.001
Xie F., Yu A., Hou D., Liu H., Ding L., Zhang S. Rapid and sensitive analysis of eight polyphenols in tobacco by rapid resolution liquid chromatography. American Journal of Analytical Chemistry, 2011, 2(8): 929-933. https://doi.org/10.4236/ajac.2011.28107
Yadav A., Chandrakar V. Experimental analysis on utilization of cigarette butts in fired clay bricks. International Journal of Scientific Research in Civil Engineering, 2022, 6(3): 129-133. Available at: https://ijsrce.com/index.php/home/article/view/IJSRCE22633
Yan N., Liu Y., Liu L., Du Y., Liu X., Zhang H., Zhang Z. Bioactivities and medicinal value of solanesol and its accumulation, extraction technology, and determination methods. Biomolecules, 2019, 9(8): 334. https://doi.org/10.3390/biom9080334
Yang W., Chen X., Li Y., Guo S., Wang Z., Yu X. Advances in pharmacological activities of terpenoids. Natural Product Communications, 2020, 15(3). https://doi.org/10.1177/1934578X20903555
Yu X., Zhou H., Ye X., Wang H. From hazardous agriculture waste to hazardous metal scavenger: Tobacco stalk biochar-mediated sequestration of Cd leads to enhanced tobacco productivity. Journal of Hazardous Materials, 2021, 413(7): 125303. https://doi.org/10.1016/j.jhazmat.2021.125303
Zareena B., Khadim A., Jeelani S., Hussain S., Ali A., Musharraf S. High-throughput detection of an alkaloidal plant metabolome in plant extracts using LC-ESI-QTOF-MS. Journal of Proteome Research, 2021, 20(8): 3826-3839. https://doi.org/10.1021/acs.jproteome.1c00111
Zeng G., Ran Y., Huang X., Li Y., Zhang M., Ding H., Ma Y., Ma H., Jin L., Sun D. Optimization of ultrasonic-assisted extraction of chlorogenic acid from tobacco waste. International Journal of Environmental Research and Public Health, 2022, 19(3): 1555. https://doi.org/10.3390/ijerph19031555
Zhang C., Yang D., Li D., Liang D., Jiang L., Huang J., Xie W., Wu D., Xi A., Wang J. Integrated microbial co-culture engineering for sustainable valorization of tobacco waste. Journal of Ecological Engineering, 2026, 27(1): 165-175. https://doi.org/10.12911/22998993/208795
Zhang W., Pan X., Fu J., Cheng W., Lin H., Zhang W., Huang Z. Phytochemicals derived from Nicotiana tabacum L. plant contribute to pharmaceutical development. Frontiers in Pharmacology, 2024, 15(3): 1-19. https://doi.org/10.3389/fphar.2024.1372456
Zhang W., Lin H., Zou M., Yuan Q., Huang Z., Pan X., Zhang W. Nicotine in inflammatory diseases: anti-inflammatory and pro-inflammatory effects. Frontiers in Immunology, 2022, 13(2): 826889. https://doi.org/10.3389/fimmu.2022.826889
Zhang X., Liu W., Xu Y., Yang L., Kong W., Rui X., Yang S., Chen Y., Miao M. Comparison of different cartridges of solid phase extraction for determination of polyphenols in tobacco by UPLC/MS/MS and multivariate analysis. Chemical Research of Chinese Universities, 2011, 27(4): 550-556.
Zhang X., Yu M., Li Y., Cheng F., Liu Y., Gao M., Liu G., Hu L., Liang Y. Effectiveness of discarded cigarette butts derived carbonaceous adsorbent for heavy metals removal from water. Microchemical Journal, 2021, 168(9): 106474. https://doi.org/10.1016/j.microc.2021.106474
Zheng Y., Cui M., Ni L., Qin Y., Li J., Pan Y., Zhang X. Heterologous expression of human metallothionein gene HsMT1L can enhance the tolerance of tobacco (Nicotiana nudicaulis Watson) to zinc and cadmium. Genes, 2022, 13(12): 2413. https://doi.org/10.3390/genes13122413
Zou X., Amrit B.K., Abu-Izneid T., Aziz A., Devnath P., Rauf A., Mitra S., Emran T.B., Mujawah A.A.H., Lorenzo J.M., Mubarak M.S., Wilairatana P., Suleria H.A.R. Current advances of functional phytochemicals in Nicotiana plant and related potential value of tobacco processing waste: A review. Biomedicine & Pharmacotherapy, 2021a, 143(11): 112191. https://doi.org/10.1016/j.biopha.2021.112191
Zou X., Bk A., Rauf A., Saeed M., Al-Awthan Y., Al-Duais M., Bahattab O., Khan M., Suleria H. Screening of polyphenols in tobacco (Nicotiana tabacum) and determination of their antioxidant activity in different tobacco varieties. ACS Omega, 2021b, 6(39): 25361-25371. https://doi.org/10.1021/acsomega.1c03275
Akhtar N., Mannan M.A.U. Mycoremediation: expunging environmental pollutants. Biotechnology Reports, 2020, 26(6): e00452. https://doi.org/10.1016/j.btre.2020.e00452
Aldeek F., Lopez V., Miller J.H. Salting-out-assisted liquid-liquid extraction method for the determination of nicotine from oral traditional and innovative tobacco products using UPLC-MS/MS. ACS Omega, 2023, 8(34): 31256-31264. https://doi.org/10.1021/acsomega.3c03474
Alhowail A. Molecular insights into the benefits of nicotine on memory and cognition. Molecular Medicine Reports, 2021, 23(6): 398-403. https://doi.org/10.3892/mmr.2021.12037
Antika L.D., Tasfiyati A.N., Hikmat H., Septama A.W. Scopoletin: a review of its source, biosynthesis, methods of extraction, and pharmacological activities. Zeitschrift für Naturforschung C, 2022, 77(7-8)c: 303-316. https://doi.org/10.1515/znc-2021-0193
Arat S.A. A review on cigarette butts: Environmental abundance, characterization, and toxic pollutants released into water from cigarette butts. Science of the Total Environment, 2024, 928(6): 172327. https://doi.org/10.1016/j.scitotenv.2024.172327
Banožić M., Aladić K., Jerković I., Jokić S. Volatile organic compounds of tobacco leaves versus waste (scrap, dust, and midrib): extraction and optimization. Journal of the Science of Food and Agriculture, 2021, 101(5): 1822-1832. https://doi.org/10.1002/jsfa.10796
Banožić M., Babić J., Jokić S. Recent advances in extraction of bioactive compounds from tobacco industrial waste-a review. Industrial Crops and Products, 2020, 144(2): 112009. https://doi.org/10.1016/j.indcrop.2019.112009
Bareschino P., Marrasso E., Roselli C. Tobacco stalks as a sustainable energy source in civil sector: Assessment of techno-economic and environmental potential. Renewable Energy, 2021, 175(9): 373-390. https://doi.org/10.1016/j.renene.2021.04.101
Beulens J.W., Booth S.L., van den Heuvel E.G., Stoecklin E., Baka A., Vermeer C. The role of menaquinones (vitamin K₂) in human health. British Journal of Nutrition, 2013, 110(8): 1357-1368. https://doi.org/10.1017/S0007114513001013
Beutel M.W., Harmon T.C., Novotny T.E., Mock J., Gilmore M.E., Hart S.C., Traina S., Duttagupta S., Brooks A., Jerde C.L., Hoh E., Van De Werfhorst L.C., Butsic V., Wartenberg A.C., Holden P.A. A review of environmental pollution from the use and disposal of cigarettes and electronic cigarettes: contaminants, sources, and impacts. Sustainability, 2021, 13(23): 12994. https://doi.org/10.3390/su132312994
Bi R., Schlaak M., Siefert E., Lord R., Connolly H. Influence of electrical fields (AC and DC) on phytoremediation of metal polluted soils with rapeseed (Brassica napus) and tobacco (Nicotiana tabacum). Chemosphere, 2011, 83(3): 318-326. https://doi.org/10.1016/j.chemosphere.2010.12.052
Boffey D. Swedish firm deploys crows to pick up cigarette butts. The Guardian. 2022. Available at: https://www.theguardian.com/environment/2022/feb/01/swedish-crows-pick-up-cigarette-butts-litter
Bonanomi G., Maisto G., De Marco A., Cesarano G., Zotti M., Mazzei P., Libralato G., Staropoli A., Siciliano A., De Filippis F., La Storia A., Piccolo A., Vinale F., Crasto A., Guida M., Ercolini D., Incerti G. The fate of cigarette butts in different environments: Decay rate, chemical changes and ecotoxicity revealed by a 5-years decomposition experiment. Environmental Pollution, 2020, 261(6): 114108. https://doi.org/10.1016/j.envpol.2020.114108
Bosco F., Mollea C. Mycoremediation in soil. In: Environmental Chemistry and Recent Pollution Control Approaches (H. Saldarriaga-Noreña, M.A. Murillo-Tovar, R. Farooq, R. Dongre, S. Riaz Eds.). InTech Open, London, UK. 2019, pp. 173-188, Print ISBN 978-1-83968-062-5, eBook (PDF) ISBN 978-1-83968-064-9. https://doi.org/10.5772/intechopen.80247
Bribi N. Pharmacological activity of alkaloids: A Review. Asian Journal of Botany, 2018, 1(2): 1-6. https://doi.org/10.63019/ajb.v1i2.467
Chaney R.L., Baklanov I.A. Phytoremediation and Phytomining: Status and Promise. In: Advances in Botanical Research (A. Cuypers, J. Vangronsveld Eds.). Academic Press, Elsevier. 2017, 83, pp. 189-221, Print ISBN 9780123815187. https://doi.org/10.1016/bs.abr.2016.12.006
Cheynier V., Comte G., Davies K., Lattanzio V., Martens S. Plant phenolics: Recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiology and Biochemistry, 2013, 72: 1-20. https://doi.org/10.1016/j.plaphy.2013.05.009
Da Silva R.P.F.F., Rocha-Santos T.A.P., Duarte A.C. Supercritical fluid extraction of bioactive compounds. TrAC Trends in Analytical Chemistry, 2016, 76(2): 40-51. https://doi.org/10.1016/j.trac.2015.11.013
Dave B., Moysa E.L., Kuźnik, A. Nicotine degradation by Trametes versicolor: Insights from diverse environmental stressors and wastewater medium. Molecules, 2025, 30(12): 2658. https://doi.org/10.3390/molecules30122658
De Fenzo A., Giordano M., Sansone L. A clean process for obtaining high-quality cellulose acetate from cigarette butts. Materials (Basel), 2020, 13(21): 4710. https://doi.org/10.3390/ma13214710
Dehaghi E., Saed S., Tahami S., Solatifar N., Taghipoor M. Using waste cigarette butt fibers as asphalt binder stabilizer in stone matrix asphalt: mechanical and environmental characterization. Journal of Materials in Civil Engineering, 2024, 36(7). https://doi.org/10.1061/JMCEE7.MTENG-17251
Dewey R.E., Xie J. Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum. Phytochemistry, 2013, 94: 10-27. https://doi.org/10.1016/j.phytochem.2013.06.002
Ding Z., Dai Y., Hao H., Pan R., Yao X., Wang Z. Anti-inflammatory effects of scopoletin and underlying mechanisms. Pharmaceutical Biology, 2009, 46(12): 854-860. https://doi.org/10.1080/13880200802367155
Dussan K., Girisuta B., Haverty D., Leahy J.J., Hayes M.H.B. Kinetics of levulinic acid and furfural production from Miscanthus giganteus. Bioresource Technology, 2013, 149(12): 216-224. https://doi.org/10.1016/j.biortech.2013.09.006
Escobar V., Gozalo G., Pérez C. Variability and performance study of the sound absorption of used cigarette butts. Materials, 2019, 12(16): 2584. https://doi.org/10.3390/ma12162584
Escobar V., González C., Gozalo G. Analysis of the influence of thickness and density on acoustic absorption of materials made from used cigarette butts. Materials, 2021, 14(16): 4524. https://doi.org/10.3390/ma14164524
Esentürk-Güzel İ., Yapar Е., Sindhu R., Kaur H., Kara B. Tobacco: Its conventional and modern dosage forms in medication. Bezmialem Science, 2022, 10(5): 655-665. https://doi.org/10.14235/bas.galenos.2021.6492
Fratiglioni L., Wang H.X. Smoking and Parkinson’s and Alzheimer’s disease: review of the epidemiological studies. Behavioural Brain Research, 2000, 113(1): 117-120. https://doi.org/10.1016/S0166-4328(00\00206-0)
Frutos M.J., Rincón-Frutos L., Valero-Cases E. Rutin. In: Nonvitamin and Nonmineral Nutritional Supplements (S.M. Nabavi, A.S. Silva Eds.). Academic Press, Elsevier. 2019, pp. 111-117, ISBN: 978-0-12-812491-8. https://doi.org/10.1016/B978-0-12-812491-8.00015-1
Ganeshpurkar A., Saluja A.K. The pharmacological potential of rutin. Saudi Pharmaceutical Journal, 2017, 25(2): 149-164. https://doi.org/10.1016/j.jsps.2016.04.025
Gao X.Y., Li X.Y., Zhang C.Y., Bai C.Y. Scopoletin: a review of its pharmacology, pharmacokinetics, and toxicity. Frontiers in Pharmacology, 2024, 15(2): 1268464. https://doi.org/10.3389/fphar.2024.1268464
Geist H.J. Tobacco and deforestation revisited. How to move towards a global land-use transition? Sustainability, 2021, 13(16): 9242. https://doi.org/10.3390/su13169242
Global tobacco market size available at (access on December 25 2025) https://www.imarcgroup.com/tobacco-processing-plant
Grebenstein P.E., Erickson P., Grace M., Kotz C.M. Anatabine, nornicotine, and anabasine reduce weight gain and body fat through decreases in food intake and increases in physical activity. Journal of Clinical Medicine, 2022, 11(3): 481. https://doi.org/10.3390/jcm11030481
Green D.S., Tongue A.D.W., Boots B., The ecological impacts of discarded cigarette butts. Trends in Ecology & Evolution, 2022, 37(2): 183-192. https://doi.org/10.1016/j.tree.2021.10.001
Guan Y., Shi H., Xu T., Zhao Y., Meng X., Liu J. Extraction and purification of nicotine from tobacco waste by a sustainable aqueous two-phase system containing recyclable hydrophilic alcohol and salt. ACS Sustainable Resource Management, 2025, 2(6): 907-914. https://doi.org/10.1021/acssusresmgt.4c00416
Gui Z.Q., Yuan X.L., Yang J., Du Y.M., Zhang, P. An updated review on chemical constituents from Nicotiana tabacum L.: Chemical diversity and pharmacological properties. Industrial Crops and Products, 2024, 214(8): 118497. https://doi.org/10.1016/j.indcrop.2024.118497
Gullón B., Lú-Chau T.A., Moreira M.T., Lema J.M., Eibes G. Rutin: a review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends in Food Science & Technology, 2017, 67(9): 220-235. https://doi.org/10.1016/J.TIFS.2017.07.008
Guo X., Li Y., Liu C., Fu Y., Gao N. Anatabine: a substitute for the medicinal activity of nicotine. European Journal of Pharmacology, 2026, 178537. https://doi.org/10.1016/j.ejphar.2026.178537
Guo Y., Hiatt E., Bonnet C., Kudithipudi C., Lewis R., Shi H., Patra B., Zhao X., de Borne F., Gilles T., Yang S., Zhang H., Zhang M., Lusso M., Berger I., Xu D., Wen L. Chapter 5 - Molecular regulation and genetic manipulation of alkaloid accumulation in tobacco plants. Studies in Natural Products Chemistry, 2021, 70: 119-149. https://doi.org/10.1016/B978-0-12-819489-8.00006-5
Hossain M., Abdulla F., Rahman A., Huq M. N. Cigarette waste: A burden to the health, environment, and economy. Ecotoxicology and Environmental Safety, 2022, 239(7): 113661. https://doi.org/10.1016/j.ecoenv.2022.113661
Hristozova G.Y., Zaprjanova P.S., Marinova S.G. Relationships between soil characteristics and concentrations of lead and zinc in soils and in oriental tobacco. Ecologia Balkanica, 2020, 12(1): 1-10. Available at: http://web.uni-plovdiv.bg/mollov/EB/2020_vol12_iss1/001-010_eb.19151.pdf
Hu R.S., Wang J., Li H., Ni H., Chen Y.F., Zhang Y.W., Xiang S.P., Li H.H. Simultaneous extraction of nicotine and solanesol from waste tobacco materials by the column chromatographic extraction method and their separation and purification. Separation and Purification Technology, 2015, 146(5): 1-7. https://doi.org/10.1016/j.seppur.2015.03.016
Ignat I., Volf I., Popa V. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry, 2011, 126(4): 1821-1835. https://doi.org/10.1016/j.foodchem.2010.12.026
Ilkaeva M., Krivtsov I., García-López E.I., Marcì G., Khainakova O., García J.R., Palmisano L., Díaz E., Ordóñez S. Selective photocatalytic oxidation of 5-hydroxymethylfurfural to 2, 5-furandicarboxaldehyde by polymeric carbon nitride-hydrogen peroxide adduct. Journal of Catalysis, 2018, 359(3): 212-222. https://doi.org/10.1016/j.jcat.2018.01.012
Ikhsanov Y.S., Nauryzbaev M., Musabekova A., Alimzhanova M., Burashev E. Study of Nicotiana tabacum L extraction, by methods of liquid and supercritical fluid extraction. Journal of Applied Engineering Science, 2019, 17(3): 338-353. https://doi.org/10.5937/jaes17-21189
Kadir A., Mohajerani A. Leachability of heavy metals from fired clay bricks incorporated with cigarette butts. In: Papers Presented to 8th International Symposium on Lowland Technology. Bandung, Indonesia, 2012, p. 872-877. Available at: https://ieeexplore.ieee.org/document/6423017
Kakoria A., Sinha-Ray S. Ultrafine nanofiber-based high efficiency air filter from waste cigarette butts. Polymer, 2022, 255(8): 125121. https://doi.org/10.1016/j.polymer.2022.125121
Kameshwar, A.K.S., Qin W. Comparative study of genome-wide plant biomass-degrading CAZymes in white rot, brown rot and soft rot fungi. Mycology, 2018, 9(2): 93-105. https://doi.org/10.1080/21501203.2017.1419296
Kaminski K., Bovet L., Hilfiker A., Laparra H., Schwaar J., Sierro N., Lang G., De Palo D., Guy P., Laszlo C., Goepfert S., Ivanov N. Suppression of pyrrolidine ring biosynthesis and its effects on gene expression and subsequent accumulation of anatabine in leaves of tobacco (N. tabacum L.). BMC Genomics, 2023, 24(1): 516-528. https://doi.org/10.1186/s12864-023-09588-8
Khan I.U., Qi S.S., Gul F., Manan S., Rono J.K., Naz M., Shi X.N., Zhang H., Dai Z.C., Du D.L. A green approach used for heavy metals ‘phytoremediation’ via invasive plant species to mitigate environmental pollution: a review. Plants, 2023, 12(4): 725. https://doi.org/10.3390/PLANTS12040725
Kheawfu K., Kaewpinta A., Chanmahasathien W, Rachtanapun P, Jantrawut P. Extraction of nicotine from tobacco leaves and development of fast dissolving nicotine extract film. Membranes (Basel), 2021, 11(6): 403. https://doi.org/10.3390/membranes11060403
Kozak K, Antosiewicz D.M. Tobacco as an efficient metal accumulator. Biometals, 2023, 36(2): 351-370. https://doi.org/10.1007/s10534-022-00431-3
Kukula-Koch W.A., Widelski J. Alkaloids. In: Pharmacognosy: Fundamentals, Applications and Strategies (S. Badal, R. Delgoda Eds.). Academic Press Elsevier. 2017, pp. 163-198. ISBN: 978-0-12-802104-0, https://doi.org/10.1016/B978-0-12-802104-0.00009-3
Kumari P., Kumar V., Kumar R., Pahuja S. Sorghum polyphenols: plant stress, human health benefits, and industrial applications. Planta, 2021, 254(3): 47. https://doi.org/10.1007/s00425-021-03697-y
Kurmus H., Mohajerani A. The toxicity and valorization options of cigarette butts. Waste Management, 2020, 104(3): 104-118. https://doi.org/10.1016/j.wasman.2020.01.011
Kurmus H., Mohajerani A., Grist S. Polycyclic aromatic hydrocarbons (PAHs) in fired clay bricks incorporating cigarette butts. Materials (Basel), 2021, 14(8): 2032. https://doi.org/10.3390/ma14082032
Lakhan S., Kirchgessner A. Anti-inflammatory effects of nicotine in obesity and ulcerative colitis. Journal of Translational Medicine, 2011, 9(129): 1-10. https://doi.org/10.1186/1479-5876-9-129
Lazarova K., Christova D., Karashanova D., Georgieva B., Marovska G., Slavov A., Babeva T. Blending approach preparation of PVA-g-PMA films with embedded “green” synthesized silver nanoparticles for acetone optical detection. Sensors, 2023, 23(6): 2941. https://doi.org/10.3390/s23062941
Lee B.J., Huang Y.C., Chen S.J., Lin P.T. Coenzyme Q10 supplementation reduces oxidative stress and increases antioxidant enzyme activity in patients with coronary artery disease. Nutrition, 2012, 28(3): 250-255. https://doi.org/10.1016/j.nut.2011.06.004
Li Y., Chen J.P., Duan L., Li S. Effect of vitamin K2 on type 2 diabetes mellitus: A review. Diabetes Research and Clinical Practice, 2018, 136(2): 39-51. https://doi.org/10.1016/j.diabres.2017.11.020
Li Z.-J., Yang D.D., Wei Z.-Y., Huang J., Chi Y.-Q., Lu Y.-X., Yin F.-W. Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications. Biotechnology for Biofuels and Bioproducts, 2024, 17(11): 144. https://doi.org/10.1186/s13068-024-02593-3
Lin X., Li Q., Pu M., Dong H., Zhang Q. Significance of nicotine and nicotinic acetylcholine receptors in Parkinson's disease. Frontiers in Aging Neuroscience, 2025, 17(3): 1535310. https://doi.org/10.3389/fnagi.2025.1535310
Liu Y., Lin M., Mu X., Qin L., Deng J., Liu Y., Wu X., He W., Pang H., Han F., Sun C., Nie X. Protective effect of solanesol in glucose-induced hepatocyte injury: Mechanistic insights on oxidative stress and mitochondrial preservation. Chemico-Biological Interactions, 2023, 383(10): 110676. https://doi.org/10.1016/j.cbi.2023.110676
Liu J., Ma G., Chen T., Hou Y., Yang S., Zhang K.-Q., Yang J. Nicotine-degrading microorganisms and their potential applications. Applied Microbiology and Biotechnology, 2015, 99(5): 3775-3785. https://doi.org/10.1007/s00253-015-6525-1
Lu P., Wu R., Xu G., Zhang B. Effective separation of nicotine from tobacco extract by silica gel. Chemical Engineering Research and Design, 2025, 217(9): 502-513. https://doi.org/10.1016/j.cherd.2025.04.030
Ludwiczuk A., Skalicka-Woźniak K., Georgiev M.I. Alkaloids. In: Pharmacognosy: Fundamentals, Applications and Strategies (S. Badal, R. Delgoda Eds.). Academic Press Elsevier. 2017, pp. 233-266. ISBN: 978-0-12-802104-0, https://doi.org/10.1016/B978-0-12-802104-0.00011-1
Luo Y., Yang Y., Schneider C., Balle T. The аnti-nociceptive effects of nicotine in humans: A systematic review and meta-analysis. Pharmaceuticals, 2023, 16(12): 1665. https://doi.org/10.3390/ph16121665
Maderuelo Sanz R., Gómez Escobar V., Meneses Rodríguez J. Potential use of cigarette filters as sound porous absorber. Applied Acoustics, 2018, 129(1): 86-91. https://doi.org/10.1016/j.apacoust.2017.07.011
Mandić N., Lalević B., Raičević V., Radojičić V. Impact of composting conditions on the nicotine degradation rate using nicotinophilic bacteria from tobacco waste. International Journal of Environmental Science and Technology, 2023, 20(7): 7787-7798. https://doi.org/10.1007/s13762-022-04405-3
Manthos G., Tsigkou K. Upscaling of tobacco processing waste management: A review and a proposed valorization approach towards the biorefinery concept. Journal of Environmental Management, 2025, 387(8): 125942. https://doi.org/10.1016/j.jenvman.2025.125942
Marinello S., Lolli F., Gamberini R., Rimini B. A second life for cigarette butts? A review of recycling solutions. Journal of Hazardous Materials, 2019, 384(2): 121245. https://doi.org/10.1016/j.jhazmat.2019.121245
Martín C., Fernández T., García R., Carrillo E., Marcet M., Galbe M., Jönsson L.J. Preparation of hydrolysates from tobacco stalks and ethanolic fermentation by Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology, 2002, 18(9): 857-862. https://doi.org/10.1023/A:1021258708507
Massadeh R., El-Elimat T., Al-Gharaibeh M., Tawaha K., Alali F. UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca. Acta Pharmaceutica, 2022, 72(1): 97-108. https://doi.org/10.2478/acph-2022-0001
Meng X., Wang X., Guan Y. Salting-out-assisted liquid-liquid extraction for nicotine from its aqueous solutions. Journal of Chemical & Engineering Data, 2022, 67(2): 453-462. https://doi.org/10.1021/acs.jced.1c00916
Missaoui B., Krafft J.M., Hamdi N., Saliba V., BenJemaa J.M., Boujday S., Bergaoui L. Valorizing industrial tobacco wastes within natural clays and chitosan nanocomposites for an ecofriendly insecticide. Waste Management, 2023, 168(8): 146-155. https://doi.org/10.1016/j.wasman.2023.05.051
Mohajerani A., Tanriverdi Y., Nguyen B., Wong K., Dissanayake H., Johnson L., Whitfield D., Thomson G., Alqattan E., Rezaei A. Physico-mechanical properties of asphalt concrete incorporated with encapsulated cigarette butts. Construction and Building Materials, 2017, 153(10): 69-80. https://doi.org/10.1016/j.conbuildmat.2017.07.091
Morales-Vera R., Cantillana J., Arto-Paz F., Hernández C., Echeverría-Vega A., Valdés C. Treatment of cigarette butts: Biodegradation of cellulose acetate by rot fungi and bacteria. Microorganisms, 2024, 12(11): 2285. https://doi.org/10.3390/microorganisms12112285
Morone A., Apte M., Pandey R.A. Levulinic acid production from renewable waste resources: Bottlenecks, potential remedies, advancements and applications. Renewable and Sustainable Energy Reviews, 2015, 51(11): 548-565. https://doi.org/10.1016/j.rser.2015.06.032
Musk A.W., De Klerk N.H. History of tobacco and health. Respirology, 2003, 8(3): 286-290. https://doi.org/10.1046/j.1440-1843.2003.00483.x
Nagargade M., Singh P., Kumar S. Commercial Crops Other than Vegetables for a Green Economy of India. In: A Critical Appraisal of India’s Self-Reliance in Agriculture (P.K. Rai, N.K. Sharma, V. K. Singh, A.K Rai Eds). Nova Science Publishers, Inc. 2023, pp. 103-131. Print ISBN: 979-8-88697-602-1. https://doi.org/10.52305/FTUU7256
Nahar N., Rahman A., Nawani N.N., Ghosh S., Mandal A. Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis Thaliana. Journal of Plant Physiology, 2017, 218(11): 121-126. https://doi.org/10.1016/j.jplph.2017.08.001
Naveed M., Hejazi V., Abbas M., Kamboh A.A., Khan G.J., Shumzaid M., Ahmad F., Babazadeh D., FangFang X., Modarresi-Ghazani F., WenHua L., XiaoHui Z. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomedicine & Pharmacotherapy, 2018, 97(1): 67-74. https://doi.org/10.1016/j.biopha.2017.10.064
Ncube E., Mhlongo M., Piater L., Steenkamp P., Dubery I., Madala N. Analyses of chlorogenic acids and related cinnamic acid derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. Chemistry Central Journal, 2014, 8(66): 1-10. https://doi.org/10.1186/s13065-014-0066-z
Nenov V. Investigation of the techno-technological parameters during production of tobacco foil through extrusion and rolling. PhD thesis by University of Food Technologies of Bulgaria, 2007 [in Bulgarian]
Newhouse P.A., Kelton M. Nicotinic systems in central nervous systems disease: degenerative disorders and beyond. Pharmacochemistry Library, 2000, 31: 91-101. https://doi.org/10.1016/S0165-7208(00\80005-7)
Nguyen B.T., Dinh D.H., Hoang N.B., Do T.T., Milham P., Hoang D.T., Cao S.T. Composted tobacco waste increases the yield and organoleptic quality of leaf mustard. Agrosystems, Geosciences & Environment, 2022, 5(3): e20283. https://doi.org/10.1002/agg2.20283
Novotny T. E., Slaughter, E. Tobacco product waste: An environmental approach to reduce tobacco consumption. Current Environmental Health Reports, 2014, 1(3): 208-216. https://doi.org/10.1007/s40572-014-0016-x
Paulista С., Ferreira E., Guimarães C., Delaqua G., Lopera H., da Silva M., Vieira C. A new proposal to reduce microplastics from cigarette butts: Production of eco-friendly fired clay bricks. Construction and Building Materials, 2024, 449(11): 138514. https://doi.org/10.1016/j.conbuildmat.2024.138514
Peševski M.D., Iliev, B.M., Živković, D.L., Popovska, V.T.J., Srbinoska, M.A., Filiposki, B.K. Possibilities for utilization of tobacco stems for production of energetic briquettes. Journal of Agricultural Sciences, 2010, 55(1): 45-54. https://doi.org/10.2298/JAS1001045P
Núñez M.P., Butler M., Majul L.M., Diorio L.A., Levin L.N., Rosenfeldt S. Colonization of cigarette butts, a toxic waste, by white rot fungi. Revista Argentina de Microbiología, 2023, 55(3): 289-290. https://doi.org/10.1016/j.ram.2023.01.003
Popova V., Ivanova T., Stoyanova A., Georgiev V., Hristeva T., Nikolova V., Docheva M., Nikolov N., Damyanova S. Phytochemicals in leaves and extracts of the variety “Plovdiv 7” of Bulgarian oriental tobacco (Nicotiana tabacum L.). Trends in Phytochemical Research, 2018, 2(1): 27-36.
Prasad K., Pathak D., Patel A., Dalwadi P., Prasad R., Patel P., Selvaraj K. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology, 2011, 10(41): 8122-8130. https://doi.org/10.5897/AJB11.394
Prokopov C., Popova V., Nikolova M., Dimov M., Ivanova T., Taneva D. Equilibrium study of Cr (VI) removal from aqueous solution by stalks from three tobacco species (Nicotiana) grown in Bulgaria. Environmental Research, Engineering and Management, 2019, 75(3): 46-54. https://doi.org/10.5755/j01.erem.75.3.23343
Puripattanavong J., Songkram C., Lomlim L., Amnuaikit T. Development of concentrated emulsion containing Nicotiana tabacum extract for use as pesticide. Journal of Applied Pharmaceutical Science, 2013, 3(11): 16-21. https://doi.org/10.7324/JAPS.2013.31104
Rahman T., Mohajerani A. Use of bitumen encapsulated cigarette butts in stone mastic asphalt. Construction and Building Materials, 2020, 261(10): 120530. https://doi.org/10.1016/j.conbuildmat.2020.120530
Rahman T., Mohajerani A., Giustozzi F. Possible recycling of cigarette butts as fiber modifier in bitumen for asphalt concrete. Materials, 2020, 13(3): 734. https://doi.org/10.3390/ma13030734
Rajesh R.O., Godan T.K., Rai A.K., Sahoo D., Pandey A., Binod P. Biosynthesis of 2, 5-furan dicarboxylic acid by Aspergillus flavus APLS-1: Process optimization and intermediate product analysis. Bioresource Technology, 2019, 284: 155-160. https://doi.org/10.1016/j.biortech.2019.03.105
Ribeiro E., Teixeira M., Gatti T., Andrade R., Suarez P. Catalytic biorefining of cigarette butts recycling waste. Chemistry, 2025, 7(3): 86. https://doi.org/10.3390/chemistry7030086
Rodgman A., Perfetti T.A. The chemical components of tobacco and tobacco smoke (Second Edition). CRC Press, Boca Raton, FL, USA, 2013, eBook ISBN: 9780429096266. https://doi.org/10.1201/b13973
Ru Q., Wang L., Li W., Wang J., Ding Y. In vitro antioxidant properties of flavonoids and polysaccharides extract from tobacco (Nicotiana tabacum L.) leaves. Molecules, 2012, 17(9): 11281-11291. https://doi.org/10.3390/molecules170911281
Ruan A., Liu C. Analysis of effect of nicotine on microbial community structure in sediment using PCR-DGGE fingerprinting. Water Science and Engineering, 2015, 8(4): 309-314. https://doi.org/10.1016/j.wse.2015.11.003
Rubio L.C.H. Estrategias para la degradación de colillas de cigarillo. PhD thesis by Pontificia Universidad Javeriana of Colombia, 2020 [in Spanish].
Shen K., Xia L., Gao X., Li C., Sun P., Liu Y., Fan H., Li X., Han L., Lu C., Jiao K., Xia C., Wang Z., Deng B., Pan F., Sun T. Tobacco as bioenergy and medical plant for biofuels and bioproduction. Heliyon, 2024, 10(13): e33920. https://doi.org/10.1016/j.heliyon.2024.e33920
Shah G., Bhatt U., Soni V. A comprehensive review on triple R eco-management strategies to reduce, reuse and recycle of hazardous cigarette butts. Heliyon, 2023, 9(6): e16642. https://doi.org/10.1016/j.heliyon.2023.e16642
Selwal N., Tabassum Z., Rahayu F., Yulia N.D., Sugiono S., Endarto O., Riajaya P.D., Djajadi D., Khamidah A., Wani A.K. Therapeutic potential and phytoremediation capabilities of the tobacco plant: Advancements through genetic engineering and cultivation techniques. Biocatalysis and Agricultural Biotechnology, 2023, 52(11): 102845. https://doi.org/10.1016/j.bcab.2023.102845
Shoji T., Hashimoto T., Saito K. Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids. Journal of Experimental Botany, 2024, 75(6): 1741-1753. https://doi.org/10.1093/jxb/erad341
Shults C.W., Oakes D., Kieburtz K., Beal M.F., Haas R., Plumb S., Juncos J.L., Nutt J., Shoulson I., Carter J., Kompoliti K., Perlmutter J.S., Reich S., Stern M., Watts R.L., Kurlan R., Molho E., Harrison M., Lew M., and the Parkinson Study Group (2002). Effects of coenzyme Q10 in early Parkinson disease: Evidence of slowing of the functional decline. Archives of Neurology, 2002, 59(10): 1541-1550. https://doi.org/10.1001/archneur.59.10.1541
Singh S., Kaur I., Kariyat R. The multifunctional roles of polyphenols in plant-herbivore interactions. International Journal of Molecular Sciences, 2021, 22(3): 1442. https://doi.org/10.3390/ijms22031442
Soleimani F., Dobaradaran S., Vazirizadeh A., Mohebbi G., Ramavandi B., De-la-Torre G., Nabipour I., Schmidt T., Novotny T., Maryamabadi A., Kordrostami Z. Chemical contents and toxicity of cigarette butts leachates in aquatic environment: A case study from the Persian Gulf region. Chemosphere, 2023, 311(2): 137049. https://doi.org/10.1016/j.chemosphere.2022.137049
Sophanodorn K., Unpaprom Y., Whangchai K., Homdoung N., Dussadee N., Ramaraj R. Environmental management and valorization of cultivated tobacco stalks by combined pretreatment for potential bioethanol production. Biomass Conversion and Biorefinery, 2022, 12(4): 1627-1637. https://doi.org/10.1007/s13399-020-00992-8
Steppuhn A., Gase K., Krock B., Halitschke R., Baldwin I.T. Nicotine’s defensive function in nature. PLOS Biology, 2004, 2(8): 1074-1080. https://doi.org/10.1371/journal.pbio.0020217
Su Y., Xian H., Shi S., Zhang C., Manik S.M.N., Mao J., Zhang G., Liao W., Wang Q., Liu H. Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk. BMC Biotechnology, 2016, 16(11): 81. https://doi.org/10.1186/s12896-016-0311-8
Suharto T., Sudirman, Ulfa M., Ningsih B., Putri K., Thamsiri P. Effect of water-methanol binary solvent system in the green synthesis of copper nanoparticles with derived from tobacco leaf extract. Acta Chimica Asiana, 2024, 7: 390-395. Available at: https://aca.unram.ac.id/index.php/ACA/article/view/197
Taylor M.A., Fraser P.D. Solanesol: Added value from Solanaceous waste. Phytochemistry, 2011, 72(11): 1323-1327. https://doi.org/10.1016/j.phytochem.2011.03.015
Teixeira M.B.H., Duarte M.A.B., Raposo Garcez L., Camargo Rubim J., Hofmann Gatti T., Suarez P.A.Z. Process development for cigarette butts recycling into cellulose pulp. Waste Management, 2017, 60(2): 140-150. https://doi.org/10.1016/j.wasman.2016.10.013
Torras-Claveria L., Jáuregui O., Codina C., Tiburcio A., Bastida J., Viladomat F. Analysis of phenolics compounds by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry in senescent and water-stressed tobacco. Plant Science, 2012, 182(1): 71-78. https://doi.org/10.1016/j.plantsci.2011.02.009
Tuzzin G., Godinho M., Dettmer A., Zattera A.J. Nanofibrillated cellulose from tobacco industry wastes. Carbohydrate Polymers, 2016, 148(9): 69-77. https://doi.org/10.1016/j.carbpol.2016.04.045
Vera-Marmanillo V., Churata R., Ortiz-Valdivia Y., Gordillo-Alarcón C., Tupayachy-Quispe D., Almirón J. Mineralizing effectiveness of Pleurotus ostreatus and Pleurotus djamor in the treatment of lignocellulosic waste from cigarette butts, Cusco (Peru). Revista De Gestão Social E Ambiental - RGSA, 2024, 18(12): e09851. https://doi.org/10.24857/rgsa.v18n12-083
Wang J., Lu D., Zhao H., Jiang B., Wang J., Ling X., Chai H., Ouyang P. Discrimination and classification of tobacco wastes by identification and quantification of polyphenols with LC-MS/MS. Journal of the Serbian Chemical Society, 2010, 75(7): 875-891. https://doi.org/10.2298/JSC091109055W
Wang J., Xu K., Zhang J., Ren G., Yang X., Zhang Z., Zhang Y., Xiao Y., Du Y. Systematic activity-oriented separation and structure-activity relationship of tobacco cembranoids. Industrial Crops and Products, 2021, 173(12): 114136. https://doi.org/10.1016/j.indcrop.2021.114136
Wang W., Yao J., Cao X. Alkaloids from tobacco leaves: Isolation, alkaloid contents, and potential application as a natural pesticide against Bactrocera dorsalis. BioResources, 2022, 17(1): 1764-1780. https://doi.org/10.15376/biores.17.1.1764-1780
Wang Y., Luo X., Chu P., Shi H., Wang R., Li J., Zheng S. Cultivation and application of nicotine-degrading bacteria and environmental functioning in tobacco planting soil. Bioresources and Bioprocessing, 2023a, 10(2): 10. https://doi.org/10.1186/s40643-023-00630-x
Wang Z., Ma L., Liu P., Luo Z., Li Z., Wu M., Xu X., Pu W., Huang P., Yang J. Transcription factor NtWRKY33a modulates the biosynthesis of polyphenols by targeting NtMYB4 and NtHCT genes in tobacco. Plant Science, 2023b, 326: 111522. https://doi.org/10.1016/j.plantsci.2022.111522
Wu X., Singh S.K., Patra B., Wang J., Pattanaik S., Yuan L. An overview of the regulation of specialized metabolism in tobacco. Current Plant Biology, 2025, 41(3): 100431. https://doi.org/10.1016/j.cpb.2024.100431
Wu Z., Yang J., Zhang Y., Wang C., Guo S., Yu Y. Growth responses, accumulation, translocation and distribution of vanadium in tobacco and its potential in phytoremediation. Ecotoxicology and Environmental Safety, 2021, 207(1): 111297. https://doi.org/10.1016/j.ecoenv.2020.111297
Xiang G., Yang H., Yang L., Zhang X., Cao Q., Miao M. Multivariate statistical analysis of tobacco of different origin, grade and variety according to polyphenols and organic acids. Microchemical Journal, 2010, 95(2): 198-206. https://doi.org/10.1016/j.microc.2009.12.001
Xie F., Yu A., Hou D., Liu H., Ding L., Zhang S. Rapid and sensitive analysis of eight polyphenols in tobacco by rapid resolution liquid chromatography. American Journal of Analytical Chemistry, 2011, 2(8): 929-933. https://doi.org/10.4236/ajac.2011.28107
Yadav A., Chandrakar V. Experimental analysis on utilization of cigarette butts in fired clay bricks. International Journal of Scientific Research in Civil Engineering, 2022, 6(3): 129-133. Available at: https://ijsrce.com/index.php/home/article/view/IJSRCE22633
Yan N., Liu Y., Liu L., Du Y., Liu X., Zhang H., Zhang Z. Bioactivities and medicinal value of solanesol and its accumulation, extraction technology, and determination methods. Biomolecules, 2019, 9(8): 334. https://doi.org/10.3390/biom9080334
Yang W., Chen X., Li Y., Guo S., Wang Z., Yu X. Advances in pharmacological activities of terpenoids. Natural Product Communications, 2020, 15(3). https://doi.org/10.1177/1934578X20903555
Yu X., Zhou H., Ye X., Wang H. From hazardous agriculture waste to hazardous metal scavenger: Tobacco stalk biochar-mediated sequestration of Cd leads to enhanced tobacco productivity. Journal of Hazardous Materials, 2021, 413(7): 125303. https://doi.org/10.1016/j.jhazmat.2021.125303
Zareena B., Khadim A., Jeelani S., Hussain S., Ali A., Musharraf S. High-throughput detection of an alkaloidal plant metabolome in plant extracts using LC-ESI-QTOF-MS. Journal of Proteome Research, 2021, 20(8): 3826-3839. https://doi.org/10.1021/acs.jproteome.1c00111
Zeng G., Ran Y., Huang X., Li Y., Zhang M., Ding H., Ma Y., Ma H., Jin L., Sun D. Optimization of ultrasonic-assisted extraction of chlorogenic acid from tobacco waste. International Journal of Environmental Research and Public Health, 2022, 19(3): 1555. https://doi.org/10.3390/ijerph19031555
Zhang C., Yang D., Li D., Liang D., Jiang L., Huang J., Xie W., Wu D., Xi A., Wang J. Integrated microbial co-culture engineering for sustainable valorization of tobacco waste. Journal of Ecological Engineering, 2026, 27(1): 165-175. https://doi.org/10.12911/22998993/208795
Zhang W., Pan X., Fu J., Cheng W., Lin H., Zhang W., Huang Z. Phytochemicals derived from Nicotiana tabacum L. plant contribute to pharmaceutical development. Frontiers in Pharmacology, 2024, 15(3): 1-19. https://doi.org/10.3389/fphar.2024.1372456
Zhang W., Lin H., Zou M., Yuan Q., Huang Z., Pan X., Zhang W. Nicotine in inflammatory diseases: anti-inflammatory and pro-inflammatory effects. Frontiers in Immunology, 2022, 13(2): 826889. https://doi.org/10.3389/fimmu.2022.826889
Zhang X., Liu W., Xu Y., Yang L., Kong W., Rui X., Yang S., Chen Y., Miao M. Comparison of different cartridges of solid phase extraction for determination of polyphenols in tobacco by UPLC/MS/MS and multivariate analysis. Chemical Research of Chinese Universities, 2011, 27(4): 550-556.
Zhang X., Yu M., Li Y., Cheng F., Liu Y., Gao M., Liu G., Hu L., Liang Y. Effectiveness of discarded cigarette butts derived carbonaceous adsorbent for heavy metals removal from water. Microchemical Journal, 2021, 168(9): 106474. https://doi.org/10.1016/j.microc.2021.106474
Zheng Y., Cui M., Ni L., Qin Y., Li J., Pan Y., Zhang X. Heterologous expression of human metallothionein gene HsMT1L can enhance the tolerance of tobacco (Nicotiana nudicaulis Watson) to zinc and cadmium. Genes, 2022, 13(12): 2413. https://doi.org/10.3390/genes13122413
Zou X., Amrit B.K., Abu-Izneid T., Aziz A., Devnath P., Rauf A., Mitra S., Emran T.B., Mujawah A.A.H., Lorenzo J.M., Mubarak M.S., Wilairatana P., Suleria H.A.R. Current advances of functional phytochemicals in Nicotiana plant and related potential value of tobacco processing waste: A review. Biomedicine & Pharmacotherapy, 2021a, 143(11): 112191. https://doi.org/10.1016/j.biopha.2021.112191
Zou X., Bk A., Rauf A., Saeed M., Al-Awthan Y., Al-Duais M., Bahattab O., Khan M., Suleria H. Screening of polyphenols in tobacco (Nicotiana tabacum) and determination of their antioxidant activity in different tobacco varieties. ACS Omega, 2021b, 6(39): 25361-25371. https://doi.org/10.1021/acsomega.1c03275
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How to Cite
KAROVA, Peya et al.
Tobacco, tobacco by-products and waste valorization opportunities.
Food Science and Applied Biotechnology, [S.l.], v. 9, n. 2, p. 462-489, oct. 2026.
ISSN 2603-3380.
Available at: <https://www.ijfsab.com/index.php/fsab/article/view/691>. Date accessed: 03 nov. 2026.
doi: https://doi.org/10.30721/fsab2026.v9.i2.691.