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Rithish Jayakumar Pranav Sasidharan Satheesh Kumar Pugalenthi Ragul Rangaraj Karthikeyan Sangapillai Ramachandra Ragunathan


Foodborne diseases remain a global concern due to their widespread impact on public health. Contaminated food can lead to outbreaks of illness that affect millions of people each year, leading to significant economic burdens and strain on healthcare systems. This study delves into the potential of cinnamon and clove essential oils to combat foodborne diseases. Essential oils extracted via the Soxhlet method using ethanol as solvent, these oils exhibit potent antibacterial, anti-inflammatory, antioxidant and anticancer properties. Gas chromatography-mass spectrometry analysis was performed to analyze the chemical constituents in both essential oils. Employing the Lipinski rule, bioactive compounds are screened and docked against a viral replication responsible protein of norovirus and the neurotoxin (botulinum toxin) of Clostridium botulinum both responsible for severe foodborne illnesses. Acetyleugenol demonstrates strong binding affinity, while the pyrimidine derivative from cinnamon sourced from Mbeya, Tanzania forms pi-sulfur interactions with both target molecules. This research emphasizes how essential oils confront foodborne diseases, granting valuable perspectives on potential compounds for extended examination and therapeutic interventions.

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Abdelhamed F.M., Abdeltawab N.F., ElRakaiby M.T., Shamma R.N., Moneib N.A. Antibacterial and anti-Inflammatory activities of Thymus vulgaris essential oil nanoemulsion onAcne vulgaris. Microorganisms, 2022, 10(9): 1874.

Aboukhalaf A., Moujabbir S., El Amraoui B., Naciri K., Kalili A., Essaih S., Rocha J., Belahsen R. Phytochemical screening, nutritional value, antioxidant and antimicrobial activities and acute toxicity of Scolymus hispanicus: A wild edible plant in Morocco. Food Science and Applied Biotechnology, 2023, 6(3): 372-382.

Adarsh A., Bharath C., Kanthesh B.M., Raghu N. Phytochemical screening and antimicrobial activity of “Cinnamon zeylanicum”. International Journal of Pharmaceutical Research and Innovations, 2020, 13(3): 22-33.

Alwan E.S., Bayoumy M.S., Mohareb R.M. Synthesis of heterocyclic compounds from camphor. Organic Communications, 2023, 16(2): 54-74.

Bai J., Li J., Chen Z., Chen X., Yang Z., Wang Z., Yang Y. Antibacterial activity and mechanism of clove essential oil against foodborne pathogens. Food Science and Technology, 2023, 173(1): 114249.

Brylinski M. Aromatic interactions at the ligand–protein interface: Implications for the development of docking scoring functions. Chemical Biology & Drug Design, 2018, 91(2): 380-390.

Chavez-Esquivel G., Cervantes-Cuevas H., Ybieta-Olvera L.F., Briones M.C., Acosta D., Cabello J.Antimicrobial activity of graphite oxide doped with silver against Bacillus subtilis, Candidaalbicans, Escherichia coli, and Staphylococcus aureus by agar well diffusion test: Synthesis and characterization. Materials Science and Engineering C, 2021, 123(4): 111934.

Daeffler K.N.M., Lester H.A., Dougherty D.A. Functionally important aromatic–aromatic and sulfur− π interactions in the D2 dopamine receptor. Journal of the American Chemical Society, 2012, 134(36): 14890-14896.

Ghadermazi R., Keramat R., Goli S.A.H. Antioxidant activity of clove (Eugenia caryophyllataThunb), oregano (Oringanumvulgare L.) and sage (Salvia officinalis L.) essential oils in various model systems. International Food Research Journal, 2017, 24(4): 1628-1635. Available at:

Gonelimali F.D., Lin J., Miao W., Xuan J., Charles F., Chen M., Hatab S.R. Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Frontiers in Microbiology, 2018, 9(7): 1639.

Gowtham K., Pranav R.J. Milk dependent hemagglutination inhibition and calcium alginate immobilization of ricin isolated from Ricinus communis L. seeds. Journal of Biological Studies, 2022, 5(4):705-713.

Gowtham K., Pranav R.J. Targeting the Bap1 protein of Vibrio cholerae for screening potent inhibitors and predicting the mutant protein stability: in silico analyses. Research Results in Biomedicine, 2024, 10(1):89-98.

Hari N., Nair V.P. FTIR spectroscopic analysis of lead extract in hexane in Jasminumazoricum L. International Journal of Scientific Research in Science and Technology, 2018, 4(8): 170-172. Print ISSN: 2395-6011, Online ISSN: 2395-602X. Available at:

Hemalakshmi S., Priyanga S., Devaki K. Fourier transform Infra-Red spectroscopy analysis of Erythrina variegate L. Journal of Pharmaceutical Sciences and Research, 2017, 9(11): 2062-2067. Available at:

Iwiński H., Różański H., Pachura N., Wojciechowska A., Gębarowski T., Szumny A. In vitro evaluation of antiprotozoal properties, cytotoxicity effect and anticancer activity of new essential-oil based phytoncide mixtures. Molecules, 2023, 28(3): 1395.

Janani K., Ajitha P., Sandhya R., Teja K.V. Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from Oreganumvulgare against Enterococcus faecalis: An in vitro study. Journal of Conservative Dentistry: JCD, 2019, 22(6): 538-543.Available at:,_minimal_inhibitory.7.aspx

Johney J., Sri S.R., Ragunathan R. Extraction of chitin and chitosan from wild Type Pleurotus spp. and its potential application-innovative approach. Journal of Pure and Applied Microbiology, 2018, 12(3): 1631-1640.

Lee H., Yoon Y. Etiological agents implicated in foodborne illness world wide. Food Science of Animal Resources, 2021, 41(1): 1-7.

Lin G.M., Chen Y.H., Yen P.L., Chang S.T. Antihyperglycemic and antioxidant activities of twig extract from Cinnamomum osmophloeum. Journal of Traditional and Complementary Medicine, 2016, 6(3): 281-288.

Manaswini N.K., Nazneen D., Rao G.B.S., Narender B., Vasudha B., Mohan M.R. Evaluation of Ocimumtenuiflorumand Syzygiumaromaticumphenolic ethereal oils for in vitro anti-inflammatory and anti-bacterial activities. Journal of Drug Delivery and Therapeutics, 2019, 9(2): 93-96.

Matulja D., Grbčić P., Bojanić K., Topić-Popović N., Což-Rakovac R., Laclef S., Šmuc T.,JovićO., Marković D., Pavelić S.K. Chemical evaluation, antioxidant,antiproliferative, anti-Inflammatoryand antibacterial activities oforganic extract and semi-purifiedfractions of the adriatic sea fan, Eunicellacavolini. Molecules, 2021, 26(19): 5751.

Ogwaro B.A., O’Gara E.A., Hill D.J., Gibson H. The Effect of combined clove and cinnamon extracts on growth and survival of Escherichia fergusonii and Salmonella typhimuriumin milk pre and post fermentation. Journal of Food Science and Nutrition Research, 2022, 5(1): 430-451.

Pettersen E.F., Goddard T.D., Huang C.C., Couch G.S., Greenblatt D.M., Meng E.C., Ferrin T.E. "UCSF Chimera - A Visualization system for exploratory research and analysis. Journal of Computational Chemistry. 2004, 25(13): 1605-1612.

Praveen A., Risshikaran V., Tamilselvan M., Rameshbabu G.N. Antimicrobial, antioxidant, antiinflammatory analysis of activity and phytochemcial screening of Cinnamomumverum, Acorouscalamus, Cinnamomum cassia, Glyceria labra. World Journal of Pharmaceutical Research, 2018, 7(18): 909-919.

Siejak P., Smułek W., Fathordobady F., Grygier A., Baranowska H.M., Rudzińska M., Masewicz Ł., Jarzębska M., Nowakowski P.T., Makiej A., Kazemian P., Drobnik P., Stachowiak B., Jarzębski M., Pratap-Singh A. Multidisciplinary studies of folk medicine “Five Thieves’ Oil” (Olejek Pięciu Złodziei)Components. Molecules, 2021, 26(10): 2931.

Trott O., Olson J.A. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. Journal of Computational Chemistry, 2010, 31(2): 455-461.

Winder N., Gohar S., Muthana M. Norovirus: An Overview of virology and preventative measures. Viruses,2022, 14(12): 2811.

Wong Y.C., Ahmad-Mudzaqqir M.Y., Wan-Nurdiyana W.A. Extraction of essential oil from Cinnamon (Cinnamomumzeylanicum). Oriental Journal of Chemistry, 2014, 30(1): 37-47.

How to Cite
PRANAV, Rithish Jayakumar et al. Exploring the therapeutic potential of cinnamon and clove essential oils for mitigating foodborne diseases: bioactivity and molecular interactions. Food Science and Applied Biotechnology, [S.l.], v. 7, n. 1, p. 122-132, mar. 2024. ISSN 2603-3380. Available at: <>. Date accessed: 17 june 2024. doi: