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Molaligne Medfu Tarekegn Mekash Teferra

Abstract

Bacillus thuringiensis (Bt.) is ubiquitous, a gram-positive and spore-forming bacterium found in natural habitats everywhere in the world. For this study, Bt. has been isolated and characterized using a variety of techniques. Twenty-one strains showed positive results for the four sets of primers including, cry1, cry2, cry3, and cry9 genes. The PCR amplified results cry1 (33.3%) were most abundant among the tested cry-type genes next to cry9 (25%), cry2 (16.6%), and cry3 (12.5%), respectively. Three strains did not amplify. Twenty-four Bt. isolates were tested for the bioassay with a third-instar diamondback moth. The mortality of this insect was not shown after treatment for 24h. However, after 48 and 72h showed 20-61% and 20-79% mortality, respectively. The four Bt. strains tested against diamondback moth larvae showed no insecticidal activity. Therefore, the isolates in this study were promising for bio-insecticidal properties for diamondback moth and plant pest control programs.

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References

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How to Cite
TAREKEGN, Molaligne Medfu; TEFERRA, Mekash. Isolation and molecular characterization of Bacillus thuringiensis strains obtained from different habitats in Northwest Ethiopia. Food Science and Applied Biotechnology, [S.l.], v. 6, n. 1, p. 134-142, mar. 2023. ISSN 2603-3380. Available at: <https://www.ijfsab.com/index.php/fsab/article/view/228>. Date accessed: 26 feb. 2024. doi: https://doi.org/10.30721/fsab2023.v6.i1.228.